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SYBR Green qPCR Master Mix (Low ROX)

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MCE SYBR Green qPCR Master Mix (Low ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which Low ROX Reference Dye is included. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :
    -20°C, 2 years. Protecting from light, and avoid repetitive freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer and ddH2O.

MCE qPCR Master Mix (Low ROX) can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Low ROX Reference Dye is included for use with instruments that require a low level of reference dye for rectify the error of fluorescence signals between different wells.

 

MCE qPCR Master Mix (Low ROX) is applied for the Real-Time PCR instrument as follows:

ABI: 7500 (Fast), ViiA 7, QuantStudio 6 and 7 Flex Systems; Stratagene: Mx3000P, Mx3005P and Mx4000; Qiagen/Corbett Rotor-Gene: 3000; Bio-Rad/MJ: Chromo4, Opticon 2 and Opticon

 

1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. Low ROX Reference Dye is included.

Storage

-20°C, 2 years.

Protecting from light, and avoid repetitive freeze-thaw cycles.

Components

Components HY-K0522-100 rxns HY-K0522-500 rxns HY-K0522-2000 rxns
SYBR Green qPCR Master Mix (Low ROX) 1 mL 1 mL × 5 1 mL × 5 × 4

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100 mg

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