1. GPCR/G Protein
    Autophagy
    Immunology/Inflammation
    Anti-infection
  2. Glucocorticoid Receptor
    SARS-CoV
    Autophagy
    Complement System
    Mitophagy
    Bacterial
    Antibiotic
  3. Dexamethasone

Dexamethasone (Synonyms: Hexadecadrol; Prednisolone F)

Cat. No.: HY-14648 Purity: 99.86%
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Dexamethasone (Hexadecadrol) is a glucocorticoid receptor agonist. Dexamethasone also significantly decreases CD11b, CD18, and CD62L expression on neutrophils, and CD11b and CD18 expression on monocytes. Dexamethasone is highly effective in the control of COVID-19 infection.

For research use only. We do not sell to patients.

Dexamethasone Chemical Structure

Dexamethasone Chemical Structure

CAS No. : 50-02-2

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Customer Review

Based on 20 publication(s) in Google Scholar

Other Forms of Dexamethasone:

Top Publications Citing Use of Products

    Dexamethasone purchased from MCE. Usage Cited in: Biochem Pharmacol. 2020 Jul 9;114145.

    After C2C12 myotubes are incubated with Dexamethasone (DEX) and S-Rg3 for 24 h, myotube levels of MuRF1 and atrogin-1 are detected using Western blot analysis.

    Dexamethasone purchased from MCE. Usage Cited in: Biochem Pharmacol. 2020 Jul 9;114145.

    Translocation of FoxO3 after treatment with S-Rg3 in DEX-treated C2C12 myotubes was investigated by immunofluorescence staining.
    • Biological Activity

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    Description

    Dexamethasone (Hexadecadrol) is a glucocorticoid receptor agonist. Dexamethasone also significantly decreases CD11b, CD18, and CD62L expression on neutrophils, and CD11b and CD18 expression on monocytes. Dexamethasone is highly effective in the control of COVID-19 infection.

    IC50 & Target

    Glucocorticoid receptor[1]

    In Vitro

    Dexamethasone (Hexadecadrol) regulates several transcription factors, including activator protein-1, nuclear factor-AT, and nuclear factor-kB, leading to the activation and repression of key genes involved in the inflammatory response[1].
    Dexamethasone potently inhibits granulocyte-macrophage colony stimulating factor (GM-CSF) release from A549 cells with EC50 of 2.2 nM. Dexamethasone (EC50=36 nM) induces transcription of the β2-receptor is found to correlate with glucocorticoid receptor (GR) DNA binding and occurred at 10-100 fold higher concentrations than the inhibition of GM-CSF release. Dexamethasone (IC50=0.5 nM) inhibits a 3×κB (NF-κB, IκBα, and I-κBβ), which is associated with inhibition of GM-CSF release[2].

    In Vivo

    It has previously been reported that treatment with Dexamethasone (Hexadecadrol) at a dose of 2×5 mg/kg efficiently inhibits lipopolysaccharide (LPS)-induced inflammation. In our experimental system, treatment with a single dose of Dexamethasone 10 mg/kg (i.p.) significantly decreases recruitment of granulocytes as well as spontaneous production of oxygen radicals compared with animals expose to LPS and injected with solvent alone (saline). The effects are statistically significant when administered both 1 h before and 1 h after inhalation of LPS. The number of granulocytes in BALF decreased to levels comparable to healthy animals (given an aerosol of water)[3].
    Rats treated with Dexamethasone consume less food and weighed less than control rats. Treated rats also weigh less than pair-fed animals though their food intake is similar. Five days of Dexamethasone injection result in a significant increase in both the liver mass (+42%) and the liver to body weight ratio (+65%). The wet weight of gastrocnemius muscle decreases 20% after 5 days of treatment, but it remains unaffected relative to body weight (g/100 g body weight), indicating that muscle weight loss paralleled body weight loss[4].

    Clinical Trial
    Molecular Weight

    392.46

    Formula

    C₂₂H₂₉FO₅

    CAS No.

    50-02-2

    SMILES
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 56 mg/mL (142.69 mM)

    Ethanol : 8.33 mg/mL (21.23 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5480 mL 12.7402 mL 25.4803 mL
    5 mM 0.5096 mL 2.5480 mL 5.0961 mL
    10 mM 0.2548 mL 1.2740 mL 2.5480 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Animal Administration
    [3][4]

    Mice[3]
    Female C57Bl/6JBom mice (age 10-12 weeks) are used in all experiments. Dexamethasone is administered as a single injection of 1 or 10 mg/kg. Dexamethasone is dissolved in saline and 400 μL are injected intraperitoneally, either 1 h before or 1 h after LPS exposure. In one experiment, N-acetylcysteine (NAC) (100 and 500 mg/kg) is injected successively every 4•5 h, starting 1 h before challenge (five injections in total). A control group of LPS-exposed animals are injected intraperitoneally with solvent alone (saline). Intratracheal administration is performed by instillation of 100 μL NAC (50, 100 or 500 mg/kg) or Dexamethasone (10 mg/kg) into the lungs of mice.
    Rats[4]
    Male Sprague-Dawley rats are used.Dexamethasone-treated rats are injected intraperitoneally once daily with Dexamethasone (1.5 mg/kg body weight) for 5 days and are allowed to feed ad libitum. The Dexamethasone dose (1.5 mg/kg/day) and the duration of treatment (5 days) are specifically chosen as this treatment induced a reproducible and marked catabolic state. Control rats received no treatment and are fed ad libitum. In order to take into account the decrease in food intake induced by Dexamethasone treatment, a third group of pair-fed rats are used. These rats are provided with the same amount of food as Dexamethasone-injected rats and are treated with a daily isovolumic intraperitoneal injection of NaCl (0.9%) for 5 days. After the final injection of Dexamethasone or NaCl, the animals are fasted overnight prior to being killed by decapitation.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.86%

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    Keywords:

    DexamethasoneHexadecadrolPrednisolone FGlucocorticoid ReceptorSARS-CoVAutophagyComplement SystemMitophagyBacterialAntibioticSARS coronavirusMitochondrial AutophagyglucocorticoidreceptorCD11bCD18CD62LneutrophilsmonocytesInhibitorinhibitorinhibit

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    Product Name:
    Dexamethasone
    Cat. No.:
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