Reconstitution Calculator

Centrifuge the tube before opening

1. During shipment, the protein may adhere to the wall or cap of the vial. Before opening the vial, please centrifuge at 10000-12000 rpm for 30 seconds to gather the protein at the bottom of the vial. If a high-speed centrifuge is not available, please centrifuge at 3000-3500 rpm for 5 mins.
2. After centrifugation, add the reconstitution buffer to the lyophilized protein powder and mix gently by pipetting. Resuspend in the reconstitution buffer to recommended concentration (no less than 100 μg/mL).
   Note: Vigorous vortexing should be avoided as it can cause protein foaming and denaturation, thereby affecting the protein activity.
3. Once reconstituted, recombinant proteins can be stored no more than a week at 2-8°C.
   For experiments with a short cycle (no more than 7 days), the recombinant protein solution can be directly added to the culture system and used up within a week. If the experimental concentration is lower than the reconstituted concentration, dilution can be done with a solution containing carrier proteins.
4. For long-term storage, the protein solution should be diluted further with carrier proteins (0.1% BSA, 5% HAS, 10% FBS or 5% trehalose), and then aliquot and stored at -20°C to -80°C.
   It is not recommended to freeze the reconstituted product directly at -20°C to -80°C. Some recombinant proteins may stick to the plastic tube wall easily, which results in a lower concentration of protein in the solution and ultimately reduces its activity. Carrier proteins can prevent products from sticking to the tube wall by pre-blocking the protein binding site. Therefore, for long-term storage, cytokines or proteins should be further diluted with the solution containing carrier proteins before making aliquots and freezing.
   Note: Avoid repeated freeze/thaw cycles. Each freeze/thaw cycle will cause denaturation or conformational changes in some proteins, thereby reducing the binding ability of antibodies and accelerating protein degradation.