CM Agarose 6FF
Based on 1 Customer Validation
MCE CM Agarose 6FF is a weak cation exchanger formed by covalently coupling carboxymethyl (CM) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.
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Storage :
1. Ensure the resin is thoroughly resuspended before use.
2. It is recommended to replace the storage buffer every 2-3 months to prevent microbial contamination due to ethanol evaporation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
- Manual (2855KB)
- COA
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Ion exchange chromatography media are high-performance purification materials composed of highly cross-linked agarose matrices covalently coupled with ion exchange ligands (e.g., DEAE, Q, CM, SP). These resins offer excellent mechanical strength, biocompatibility, chemical stability, and superior flow and separation characteristics. They are suitable for a wide range of cation or anion exchange purification processes and are widely applied in the separation and purification of recombinant proteins, antibodies, enzymes, nucleic acids, as well as in vaccine production, diagnostic raw material preparation, and biopharmaceutical development.
MCE CM Agarose 6FF is a weak cation exchanger formed by covalently coupling carboxymethyl (CM) groups to agarose. It features high binding capacity, high specificity, and excellent ligand stability. It is suitable for both laboratory research and large-scale industrial purification processes.
Each 1 mL of total volume contains 0.5 mL of resin. Thoroughly resuspend the resin before use and mix well before aliquoting.
The specifications of the product correspond to the actual resin volume, with the resin content of 50%.
Protocol
Recommended Buffers (Not Provided)
| Buffer | Composition |
|---|---|
| Binding/Wash Buffer | 20 mM PB |
| Elution Buffer | 20 mM PB, 1-2 M NaCl |
| Storage Buffer | 0.2 M sodium acetate, 20% ethanol |
Notes: a. Prepare all buffers using ultrapure water and sterilize them by filtration through a 0.45 μm or 0.22 μm membrane;
b. The formulations are provided for reference and may be adjusted according to experimental requirements. As a general principle, load the sample under low-salt conditions and elute under high-salt conditions;
c. The pH of the binding/wash buffer is typically set 1-3 units below the isoelectric point of the target protein.
Sample Preparation
1. Sample Filtration: Before purification, filter the sample through a 0.22 μm or 0.45 μm membrane.
2. Condition Adjustment: Adjust the sample pH and conductivity to match those of the equilibration buffer by dilution, ultrafiltration, or buffer exchange (desalting).
Protein Purification
1. Column Packing: Pack CM Agarose 6FF into a suitable chromatography column and connect the column to the chromatography system.
2. Column Equilibration: Equilibrate the column with 5 column volumes of binding/wash buffer. Repeat the procedure 2-3 times.
3. Sample Loading: Load the sample using a pump or sample loop and collect the flow-through.
Notes: a. Select an appropriate resin volume according to the protein amount and do not exceed the binding capacity of the column;
b. Ensure that the sample has suitable ionic strength and pH;
c. High sample viscosity or large volumes may increase column backpressure.
4. Washing: Wash the column with 10-20 column volumes of binding/wash buffer to remove nonspecifically bound proteins. Collect the wash fractions until the UV absorbance baseline stabilizes.
5. Elution: Elute the target protein with 5-10 column volumes of elution buffer and collect the eluate in separate fractions.
6. Regeneration: Wash the column thoroughly with 5-10 column volumes of elution buffer, followed by 10-20 column volumes of ddH2O.
Optional: Cleaning-in-Place (CIP)
1. Removal of Tightly Bound Nonspecific Proteins: Wash with 5-10 column volumes of 2 M NaCl, followed by 10-20 column volumes of ddH2O.
2. Removal of Precipitates and Strongly Hydrophobic Contaminants: Wash with 3-5 column volumes of 1 M NaOH, followed by 10-20 column volumes of ddH2O.
3. Removal of Lipid Contaminants: Wash with 3-5 column volumes of 70% ethanol or 30% isopropanol, followed by 10-20 column volumes of ddH2O.
Storage
After final cleaning, equilibrate the column with 5-10 column volumes of storage buffer, disconnect the column, and store at 4°C.
Storage
1. Ensure the resin is thoroughly resuspended before use.
2. It is recommended to replace the storage buffer every 2-3 months to prevent microbial contamination due to ethanol evaporation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
Attention
1. Ensure the resin is thoroughly resuspended before use.
2. It is recommended to replace the storage buffer every 2-3 months to prevent microbial contamination due to ethanol evaporation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
| Components | HY-K0257-25 mL | HY-K0257-50 mL | HY-K0257-100 mL |
|---|---|---|---|
| CM Agarose 6FF | 25 mL | 50 mL | 100 mL |
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