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  2. Transfer Buffer (Semi Dry) Powder (1 L of 1×)

Transfer Buffer (Semi Dry) Powder (1 L of 1×) 

Cat. No.: HY-K1028
Manual SDS

MCE Transfer Buffer (Semi Dry) Powder (1 L of 1×) consists of Tris, Glycine and SDS, and is suitable for Western Blot semi-dry electrophoresis.

Transfer Buffer (Semi Dry) Powder (1 L of 1×)
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  • Description

  • Storage

  • Protocol

  • components

  • Documentation

Description
& Advantages

MCE Transfer Buffer (Semi Dry) Powder (1 L of 1×) consists of Tris, Glycine and SDS. Each pouch can be diluted to 1 L 1× transfer buffer, in which the concentration of Tris is 48 mM, the concentration of Glycine is 39 mM and the concentration of SDS is 0.04%. This product is suitable for Western Blot semi-dry electrophoresis. This product provides a convenient way to make transfer buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1.   Add 1 pouch of powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2.   Add 200 mL methanol to the solution in step 1, and then add distilled water until the total volume is 1 L. The final solution is 1× transfer buffer.

Note : The newly prepared 1× transfer buffer can be stored for 2 weeks at room temperature.

components
Components HY-K1028-20 pouches HY-K1028-100 pouches
Transfer Buffer (Semi Dry) Powder (1 L of 1×) 20 pouches 5 × 20 pouches
Documentation

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Product Name:
Transfer Buffer (Semi Dry) Powder (1 L of 1×)
Cat. No.:
HY-K1028
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MCE Japan Authorized Agent: