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PDGFR beta Antibody (YA1925)(PBS only)

(Synonyms: PDGFRB; PDGFR; PDGFR1; PDGFRβ; Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-deri)

PDGFR beta Antibody (YA1925) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDGFR beta.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Experimental Validation Results for PDGFR beta Antibody (YA1925)(PBS only)
  • PDGFR beta Antibody (YA1925)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:50-1:200 1:50-1:100 1:20-1:50 1:20

Product Details

Description

PDGFR beta Antibody (YA1925) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDGFR beta.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 190 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 124 kDa
Immunogen

A synthetic peptide of human PDGFR beta aa1094-1106.

Purification

Affinity Purified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PDGFR beta Antibody (YA1925)(PBS only)
    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), U-87MG (lane 2, 15 μg), 3T3 (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using PDGFR beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    PDGFR beta is a PDGF Receptor beta is a tyrosine-protein kinase that functions as a cell-surface receptor for homodimeric PDGFB and PDGFD, as well as heterodimers formed by PDGFA and PDGFB. It plays essential roles in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration. Critical for blood vessel development, it promotes the proliferation, migration, and recruitment of pericytes and smooth muscle cells to endothelial cells. It also contributes to vascular smooth muscle cell migration and neointima formation at injury sites, and is required for normal cardiovascular system development, pericyte (mesangial cell) recruitment in kidney glomeruli, and glomerular capillary network formation. The receptor induces actin cytoskeleton rearrangement and membrane ruffle formation. Ligand binding activates multiple signaling cascades, phosphorylating PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1, and NCK1, thereby regulating downstream effectors such as diacylglycerol, inositol 1,4,5-trisphosphate, Ca2+ mobilization, protein kinase C, AKT1, HRAS, RAF1, and MAPK1/ERK2/MAPK3/ERK1. Receptor signaling is downregulated by phosphatase-mediated dephosphorylation and rapid internalization. by similar: It also promotes phosphorylation and activation of SRC family kinases, PDCD6IP/ALIX, and STAM.

  • SwissProt ID

    P09619

  • Gene ID
    5159 [NCBI]
  • Synonyms

    PDGFRB; PDGFR; PDGFR1; PDGFRβ; Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-deri

PDGFR beta Antibody (YA1925)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), U-87MG (lane 2, 15 μg), 3T3 (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using PDGFR beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PDGFR beta Antibody (YA1925)(PBS only) Product Image
    1/16
  • Experimental Validation Results for PDGFR beta Antibody (YA1925)(PBS only)
  • PDGFR beta Antibody (YA1925)(PBS only) Product Image
  • Experimental Validation Results for PDGFR beta Antibody (YA1925)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), U-87MG (lane 2, 15 μg), 3T3 (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using PDGFR beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PDGFR beta Antibody (YA1925)(PBS only)
  • PDGFR beta Antibody (YA1925)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), U-87MG (lane 2, 15 μg), 3T3 (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using PDGFR beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PDGFR beta Antibody (YA1925)(PBS only) Product Image WB