Plant Tissue Direct PCR Kit (with Dye) 

MCE Plant Tissue Direct PCR Kit (with Dye) is specifically designed for direct amplification from various plant leaf samples. The kit enables rapid release of genomic DNA from plant tissues (such as rice, maize, tobacco, rapeseed, etc.) in a single step. The resulting lysate can be directly used for downstream PCR amplification without the need for conventional DNA extraction and purification, thereby significantly simplifying the experimental workflow.

The kit is supplied with a ready-to-use PCR master mix containing all components required for amplification, including Taq DNA polymerase, dNTPs, an optimized buffer system, and tracking dyes. Only primers and template need to be added to initiate the PCR reaction. The amplified products can be directly analyzed by agarose gel electrophoresis without the addition of loading buffer, effectively reducing operational complexity and the risk of contamination. It is suitable for applications such as transgenic plant identification and plant genotyping.

Features of MCE Plant Tissue Direct PCR Kit (with Dye):

1. Low sample requirement: Efficient lysis can be achieved using only a small amount of plant tissue (as little as ~1 mm of leaf tissue).

2. High Specificity and Efficiency: Optimized Taq polymerase system with strong template affinity and high amplification specificity, ideal for genotyping and transgenic analysis.

3. User-Friendly Operation: The master mix contains tracking dyes, allowing PCR products to be directly loaded for electrophoresis.

Lysis Buffer: 4°C, 1 year.

Neutralization Buffer, Direct PCR Master Mix (2×, with Dye): -20°C, 1 year. Avoid repeated freeze-thaw cycles.

1. Freshly collected leaf tissue is recommended. For samples stored long-term, keep them at −80°C and avoid repeated freeze–thaw cycles to prevent genomic DNA degradation, which may adversely affect PCR amplification efficiency and result reliability.

2. Young leaf tissue is preferred. If mature leaves must be used, avoid sampling from the main vein region.

3. This product is for R&D use only, not for drug, household, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.