1. MCE Kits
  2. Cell Biology
  3. Cell Analysis
  4. Phalloidin Dye Conjugates
  5. AMCA Phalloidin

AMCA Phalloidin 

Cat. No.: HY-K0901
Manual SDS

MCE AMCA Phalloidin is Phalloidin conjugated to the fluorescent dye AMCA. Phalloidin binds F-actins with high selectivity while AMCA provides stable and bright blue fluorescence.

AMCA Phalloidin
Size Price Stock Quantity
300T USD 432 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE Phalloidin is a mushroom-derived toxin which can be used to specifically label F-actins (actin filaments) of the cytoskeleton. It binds to all variants of actin filaments in many different species of animals and plants. Typically, Phalloidin is used conjugated to a fluorescent dye. Phalloidin binds F-actins with high selectivity while AMCA provides stable and bright blue fluorescence.

AMCA Phalloidin is 1000× stock solution in DMSO which is convenient for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.

Storage

The solutions should be stable for at least 6 months if store at -20°C.

Protecting from light, and avoid freeze/thaw cycles.

Protocol

1. Prepare 1× AMCA Phalloidin working solution:

Add 1 μL of 1000× AMCA Phalloidin DMSO solution to 1 mL of PBS containing with 1% BSA.

Note: a. Different cell types might be stained differently. The concentration of AMCA Phalloidin working solution should be prepared accordingly.

b. 1% bovine serum albumin (BSA) is used to reduce nonspecific background staining.

2. Stain the cells:

2.1 Wash cells 2-3 times with PBS. Fix the cells in 3.7% methanol-free formaldehyde solution in PBS for 10-30 minutes at room temperature.

Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.

2.2 Wash the fixed cells 2-3 times in PBS.

2.3 Add 0.1% Triton X-100 in PBS into fixed cells for 3-5 minutes to increase permeability. Wash the cells 2-3 times in PBS.

2.4 Add 100 μL/well (96-well plate) of AMCA Phalloidin working solution into the fixed cells, and stain the cells at room temperature for 20 to 90 minutes.

2.5 Rinse cells gently with PBS 2-3 times to remove excess AMCA Phalloidin.

2.6 Run fluorescence microscope or other equipments at desired Ex/Em wavelengths (AMCA Phalloidin, Ex/Em=344/432 nm).

Components
Cat. No. Product Name Ex (nm) Em (nm) Size Package
HY-K0901 AMCA Phalloidin 344.0 432.0 300 T 30 μL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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AMCA Phalloidin
Cat. No.:
HY-K0901
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