AMCA Phalloidin
Based on 1 Customer Validation
MCE AMCA Phalloidin is Phalloidin conjugated to the fluorescent dye AMCA. Phalloidin binds F-actins with high selectivity while AMCA provides stable and bright blue fluorescence.
-
Storage :
The solutions should be stable for at least 6 months if store at -20°C.
Protecting from light, and avoid freeze/thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE Phalloidin is a mushroom-derived toxin which can be used to specifically label F-actins (actin filaments) of the cytoskeleton. It binds to all variants of actin filaments in many different species of animals and plants. Typically, Phalloidin is used conjugated to a fluorescent dye. Phalloidin binds F-actins with high selectivity while AMCA provides stable and bright blue fluorescence.
AMCA Phalloidin is 1000× stock solution in DMSO which is convenient for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
Protokoll
1. Prepare 1× AMCA Phalloidin working solution:
Add 1 μL of 1000× AMCA Phalloidin DMSO solution to 1 mL of PBS containing with 1% BSA.
Note: a. Different cell types might be stained differently. The concentration of AMCA Phalloidin working solution should be prepared accordingly.
b. 1% bovine serum albumin (BSA) is used to reduce nonspecific background staining.
2. Stain the cells:
2.1 Wash cells 2-3 times with PBS. Fix the cells in 3.7% methanol-free formaldehyde solution in PBS for 10-30 minutes at room temperature.
Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
2.2 Wash the fixed cells 2-3 times in PBS.
2.3 Add 0.1% Triton X-100 in PBS into fixed cells for 3-5 minutes to increase permeability. Wash the cells 2-3 times in PBS.
2.4 Add 100 μL/well (96-well plate) of AMCA Phalloidin working solution into the fixed cells, and stain the cells at room temperature for 20 to 90 minutes.
2.5 Rinse cells gently with PBS 2-3 times to remove excess AMCA Phalloidin.
2.6 Run fluorescence microscope or other equipments at desired Ex/Em wavelengths (AMCA Phalloidin, Ex/Em=344/432 nm).
Speicherung
The solutions should be stable for at least 6 months if store at -20°C.
Protecting from light, and avoid freeze/thaw cycles.
Components
| Cat. No. | Product Name | Ex (nm) | Em (nm) | Size | Package |
|---|---|---|---|---|---|
| HY-K0901 | AMCA Phalloidin | 344.0 | 432.0 | 300 T | 30 μL |
Please enter the correct Lot Number
