1. MCE Kits
  2. Cytotoxicity LDH Assay Kit

Cytotoxicity LDH Assay Kit 

Cat. No.: HY-K1090
Manual SDS

MCE Cytotoxicity LDH Assay Kit can detect LDH activity in the culture supernatant, which is indicative of cytotoxicity. Meanwhile viable cell number can be detected by CCK8 Kit (HY-K0301).

Cytotoxicity LDH Assay Kit
Size Price Stock Quantity
100 T USD 106 In-stock
Estimated Time of Arrival: December 31
500 T USD 255 In-stock
Estimated Time of Arrival: December 31

* Please select Quantity before adding items.

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

The loss of cell membrane integrity due to cell death or membrane damage results in release of certain soluble, cytosolic enzymes. Therefore, acute cytotoxicity can be measured by measuring activity of these enzymes released into the culture supernatant. Lactate dehydragenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

LDH oxidizes lactate to generate NADH, which then reacts with certain dye to generate yellow color. The intensity of the generated color correlates directly with the cell number lysed, which is indicative of cytotoxicity. LDH activity can be easily quantified by spectrophotometer or plate reader at 490 nm.

Fig 1. Schematic of MCE Cytotoxicity LDH Assay Kit.

MCE Cytotoxicity LDH Assay Kit can be directly added to cells to detect LDH activity in the culture supernatant (one-step, direct method). Or you can separate the culture supernatant from cell and use this kit to detect LDH activity (indirect method). The separated cells can be used for other experiments.

Fig 2. Detect LDH activity by direct and indirect method.

Storage

Stored at -20°C protecting from light, and is stable for up to 12 months.

Components
Components HY-K1090-100T HY-K1090-500T
Working Solution 5.5 mL 27.5 mL
Stop Solution 5.5 mL 27.5 mL
Lysis Solution 1.1 mL 5.5 mL
Documentation

Your Recently Viewed Products:

Bulk Inquiry

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Cytotoxicity LDH Assay Kit
Cat. No.:
HY-K1090
Quantity:
MCE Japan Authorized Agent: