1. MCE Kits
  2. Cell Biology
  3. Cell Analysis
  4. Cell Proliferation and Cytotoxicity
  5. Cytotoxicity LDH Assay Kit

Cytotoxicity LDH Assay Kit 

Cat. No.: HY-K1090
Manual SDS

MCE Cytotoxicity LDH Assay Kit can detect LDH activity in the culture supernatant, which is indicative of cytotoxicity. Meanwhile viable cell number can be detected by CCK8 Kit (HY-K0301).

Cytotoxicity LDH Assay Kit
Size Price Stock Quantity
Free Sample (10T)   Apply Now  
100 T USD 106 In-stock
500 T USD 255 In-stock

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  • Description

  • Storage

  • Components

  • Documentation

& Advantages

The loss of cell membrane integrity due to cell death or membrane damage results in release of certain soluble, cytosolic enzymes. Therefore, acute cytotoxicity can be measured by measuring activity of these enzymes released into the culture supernatant. Lactate dehydragenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

LDH oxidizes lactate to generate NADH, which then reacts with certain dye to generate yellow color. The intensity of the generated color correlates directly with the cell number lysed, which is indicative of cytotoxicity. LDH activity can be easily quantified by spectrophotometer or plate reader at 490 nm.

Fig 1. Schematic of MCE Cytotoxicity LDH Assay Kit.

MCE Cytotoxicity LDH Assay Kit can be directly added to cells to detect LDH activity in the culture supernatant (one-step, direct method). Or you can separate the culture supernatant from cell and use this kit to detect LDH activity (indirect method). The separated cells can be used for other experiments.

Fig 2. Detect LDH activity by direct and indirect method.


Stored at -20°C protecting from light, and is stable for up to 12 months.

Components HY-K1090-100T HY-K1090-500T
Working Solution 5.5 mL 27.5 mL
Stop Solution 5.5 mL 27.5 mL
Lysis Solution 1.1 mL 5.5 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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