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Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO)

246 Cited Publications
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Based on 246 publication(s) in Google Scholar

Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) is a mixture of multiple phosphatase inhibitors that effectively inhibit serine/threonine phosphatases and alkaline phosphatases, and is used to preserve the phosphorylation status of proteins.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :
    -20℃, 2 years.
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO)
  • Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) Product Image
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Description & Advantages

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases.

MCE Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) is a mixture of multiple phosphatase inhibitors that effectively inhibit serine/threonine phosphatases and alkaline phosphatases, and is used to preserve the phosphorylation status of proteins. This product can be applied in Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, Kinase assay and etc.

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Protocol

Thaw at room temperature, add the inhibitor mixture to the lysate at 1:100 (v/v), and mix thoroughly.

Storage

-20℃, 2 years.

Components

Cat. No. Product Name Package
HY-K0023-1 mL Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) 1 mL
HY-K0023-10 mL Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) 1 mL × 10
HY-K0023-50 mL Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) 1 mL × 50
HY-K0023-100 mL Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) 1 mL × 100

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  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO)

    Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Mar 11;14(5):2246-2264. [Abstract]

    Representative immunoblots and quantification of cell-surface CD36 in heart lysates from both control and Atp6v0d1AKOheart tissues.

Assay Types
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO)
    1/16

    Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Mar 11;14(5):2246-2264. [Abstract]

    Representative immunoblots and quantification of cell-surface CD36 in heart lysates from both control and Atp6v0d1AKOheart tissues.

  • Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) Product Image
    1/16
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO)
  • Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) Product Image