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  2. Phosphatase Inhibitor Cocktail III

Phosphatase Inhibitor Cocktail III  (100× in DMSO)

Cat. No.: HY-K0023
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Phosphatase Inhibitor Cocktail III effectively inhibits serine/threonine and alkaline phosphatases.

Phosphatase Inhibitor Cocktail III
Size (Pre-dissolved in DMSO) Price Stock Quantity
Free Sample   Apply now  
1  mL USD 55 In-stock
Estimated Time of Arrival: December 31
1  mL × 10 USD 395 In-stock
Estimated Time of Arrival: December 31
1  mL × 50 USD 1840 In-stock
Estimated Time of Arrival: December 31
1  mL × 100 USD 2765 In-stock
Estimated Time of Arrival: December 31

* Please select Quantity before adding items.

Top Publications Citing Use of Products

Customer Validation

  • Protein: Bcl-2

    Cell Line: thyroid cancer cell line 8505C

    MCE Protease Inhibitor Cocktail provides Bcl-2 protection in thyroid cancer cell line 8505C, and is most effective after 6-12 hours incubation at room temperature.

  • Protein: ERK1/2

    Cell Line: K1 cell line

    MCE Protease Inhibitor Cocktail (mini-Tablet) protects ERK1/2 in K1 cell line, and is most effective after 6-12 hours incubation at room temperature.

MCE Protease Inhibitor Cocktail provides Bcl-2 protection in thyroid cancer cell line 8505C, and is most effective after 6-12 hours incubation at room temperature.

 

MCE Protease Inhibitor Cocktail (mini-Tablet) protects ERK1/2 in K1 cell line, and is most effective after 6-12 hours incubation at room temperature.

 
  • Description

  • Price Comparison

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Each vial contains a 1 ml solution with the following components: 500 µM Cantharidin, 2.5 mM (-)-p-Bromotetramisole oxalate, and 1 µM Calyculin A form Discodermia calyx.

Phosphatase Inhibitor Cocktail III has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.

Publications
Price Comparison
Phosphatase Inhibitor Cocktail III Price Comparison
Storage
The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.
Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components
Ingredient M.W. 100x Conc. Target
(-)-p-Bromolevamisole oxalate 373.2 2.5 mM Alkaline phosphatases
Cantharidin 196.2 500 µM protein phosphatase 2A(PP2A)
Calyculin A 1009.2 1 µM protein phosphatases 1 and 2A(PP1 and PP2A)
Documentation

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Product Name:
Phosphatase Inhibitor Cocktail III
Cat. No.:
HY-K0023
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