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  2. Protein Biology
  3. Protein Sample Preparation
  4. Protease Inhibitor Cocktail
  5. Protease Inhibitor Cocktail

Protease Inhibitor Cocktail  (EDTA-Free,100× in DMSO)

Cat. No.: HY-K0010
Manual COA SDS

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.

Protease Inhibitor Cocktail
Size (Pre-dissolved in DMSO) Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 35 In-stock
Estimated Time of Arrival: December 31
1 mL × 10 USD 250 In-stock
Estimated Time of Arrival: December 31
1 mL × 50 USD 1000 In-stock
Estimated Time of Arrival: December 31
1 mL × 100 USD 1500 In-stock
Estimated Time of Arrival: December 31

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  • Description

  • Price Comparison

  • Storage

  • Protocol

  • Components

  • Documentation

& Advantages

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.

Protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. This cocktail is supplied as a ready-to-use solution in DMSO.

Price Comparison
Protease Inhibitor Cocktail Price Comparison
The product should be stored at -20°C, and is stable for at least 12 months.

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts), before assaying.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Ingredient M.W. 100x Conc. Target
AEBSF 239.5 104 mM Serine proteases
Aprotinin 6511.5 80 μM Serine proteases
Bestatin 308.38 5 mM Aminopeptidases
E-64 357.4 1.5 mM Cysteine proteases
Leupeptin 475.6 2 mM Serine and cysteine proteases
Pepstatin A 685.9 1.5 mM Aspartic proteases
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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