An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA

  • STAR Protoc. 2021 Apr 27;2(2):100504. doi: 10.1016/j.xpro.2021.100504.
Lingli He  1 ,  Wentao Yu  1 ,  Wenxiang Zhang  1 ,  Lei Zhang  1  2  3
Affiliations
  • 1. State Key Laboratory of Cell Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
  • 2. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • 3. School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.
PMID: 33997818 DOI: 10.1016/j.xpro.2021.100504
Abstract

Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP. Here, we provide an optimized two-step ChIP protocol to quantify the in vivo associates of multiple proteins with the same DNA regulatory element. For complete details on the use and execution of this protocol, please refer to He et al. (2020).

Keywords
Chromatin immunoprecipitation (ChIP); Molecular Biology.
Products