In Vitro Angiogenesis Basement Membrane Matrix (Ready-to-Use)
Based on 1 Customer Validation
MCE In Vitro Angiogenesis Basement Membrane Matrix (Ready-to-Use) is formulated in high-glucose DMEM and contains 50 μg/mL gentamicin. With an optimized formulation and working concentration, it requires no additional dilution or preparation and can be directly used for in vitro tube formation assays with HUVECs and other endothelial cells, supporting the establishment of in vitro angiogenesis models and related research.
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Storage :
-20°C, 1 year. Shipping with dry ice.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Basement Membrane Matrix is a natural basement membrane preparation extracted from a mouse tumor rich in extracellular matrix (ECM) proteins. It mimics the in vivo cellular microenvironment and provides structural support for cell growth. The matrix contains abundant ECM components, including: Laminin, Collagen IV, Entactin/Nidogen, Heparan Sulfate Proteoglycans (HSPGs). It also contains various naturally occurring growth factors, including: Insulin-like Growth Factor-1 (IGF-1), Transforming Growth Factor-β (TGF-β), Vascular Endothelial Growth Factor (VEGF), Epidermal Growth Factor (EGF), Basic Fibroblast Growth Factor (bFGF).
MCE In Vitro Angiogenesis Basement Membrane Matrix (Ready-to-Use) is formulated in high-glucose DMEM and contains 50 μg/mL gentamicin. With an optimized formulation and working concentration, it requires no additional dilution or preparation and can be directly used for in vitro tube formation assays with HUVECs and other endothelial cells, supporting the establishment of in vitro angiogenesis models and related research.
The convenient ready-to-use format allows immediate use after opening, minimizing hands-on preparation, streamlining workflow, improving experimental efficiency, and ensuring excellent batch-to-batch consistency and reproducibility.
Protocol
1. Matrix Gel Thawing: Remove the matrix gel, place it on ice, and allow it to thaw slowly at 4°C before use. Avoid repeated freeze-thaw cycles during the thawing process.
2. Matrix Gel Coating: Add an appropriate amount of matrix gel to the cell culture plate to evenly cover the entire growth surface. Add the gel slowly and gently tilt or swirl the plate to ensure uniform distribution and avoid bubble formation. Recommended volumes for different culture vessels are listed below.
| Plate Type | Growth Area | Recommended Volume |
|---|---|---|
| 96-well plate | ~ 0.32 cm2 | 50 μL |
| 48-well plate | ~ 0.7 cm2 | 100 μL |
| 24-well plate | ~ 2 cm2 | 200 μL |
| 12-well plate | ~ 4.5 cm2 | 500 μL |
| 6-well plate | ~ 9.6 cm2 | 1 mL |
3. Matrix Gel Polymerization: Incubate the coated plate at 37°C for at least 30 min until the matrix gel is fully polymerized before use.
4. Cell Seeding: Resuspend the cells in Endothelial Cell Medium and slowly add an appropriate volume of the cell suspension along the wall of the culture vessel to ensure even distribution of the cells over the matrix gel surface.
Note: It is recommended to optimize the cell seeding density through preliminary experiments to achieve optimal tube formation.
5. Culture and Analysis: Carefully transfer the culture plate to a cell culture incubator and continue incubation. At the designated time point, remove the plate and observe tube formation under a microscope for further analysis.
Note: It is recommended to begin observing tube formation approximately 4 h after cell seeding.
Storage
-20°C, 1 year.
Shipping with dry ice.
Attention
1. This product has been optimized for ready-to-use applications. Further dilution is not recommended, as it may affect coating performance.
2. Please bury this product with packaging in ice and thaw in the 4°C refrigerator. After thawing, make aliquots and keep them frozen.
3. The matrix readily gels above 10°C; maintain low temperature throughout handling.
4. Pre-cool all consumables (pipette tips, tubes, culture plates, etc.) before use.
5. Avoid holding the lower portion of containers containing matrix to prevent localized warming and gelation.
6. If the matrix becomes viscous or partially gelled due to warming, place it on ice and incubate at 4°C for 1-2 h until fluidity is restored.
7. During thawing, ensure that the storage container remains tightly sealed to prevent leakage or tube rupture.
8. This product is for R&D use only, not for drug, household, or other uses.
9. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K6026-5 mL | HY-K6026-10 mL |
|---|---|---|
| In Vitro Angiogenesis Basement Membrane Matrix (Ready-to-Use) | 5 mL | 10 mL |
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