CEPT Cocktail Plus (1000×)
Based on 1 Customer Validation
MCE CEPT Cocktail Plus (1000×) is a composite supplement specifically formulated for pluripotent stem cell culture. Through synergistic effects, it inhibits oxidative damage, blocks apoptotic pathways, and regulates protein translation, significantly reducing cellular stress levels through multiple targets. This dramatically improves the survival rate and cloning efficiency of pluripotent stem cells.
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Storage :
-20°C, 2 years.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE CEPT Cocktail Plus (1000×) is a composite supplement specifically formulated for pluripotent stem cell culture. It contains three key small molecules-Chroman 1 (antioxidant), Emricasan (caspase inhibitor), and Trans-ISRIB (integrated stress response modulator)—and is further supplemented with Polyamine Solution, L-ascorbic acid phosphate (LAA), niacin, cysteine, and other auxiliary components.
Through synergistic actions that suppress oxidative damage, block apoptotic signaling, and regulate protein translation, MCE CEPT Cocktail Plus (1000×) effectively reduces cellular stress via multiple pathways, resulting in markedly improved viability and cloning efficiency of pluripotent stem cells. In addition, CEPT Cocktail Plus (1000×) enhances culture stability during routine passaging, promotes post-thaw recovery following ultra-low-temperature cryopreservation, and supports efficient formation of embryoid bodies and diverse organoids (such as intestinal and brain organoids).
Protocol
1. Thaw CEPT Cocktail Plus (1000×) at 4°C in advance.
Note: a. Aliquot as needed to minimize repeated freeze/thaw cycles.
b. Due to localized concentrations of polyamines, a milky or flocculent precipitate may appear during storage at 4°C. Mix thoroughly before use.
2. Add CEPT Cocktail Plus (1000×) to the culture medium at a 1:1000 ratio. Example: Add 10 μL of CEPT Cocktail Plus (1000×) to 10 mL of culture medium.
3. Filter the prepared complete medium through a 0.22 μm membrane before use.
Note: It is recommended to use the medium immediately after preparation; long-term storage is not recommended.
Storage
-20°C, 2 years.
Attention
1. This product is sterile and should be handled using aseptic techniques. It is recommended to aliquot and store to avoid contamination.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
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