Exosome Protein Lysis Buffer
Based on 2 publication(s) in Google Scholar
MCE Exosome Protein Lysis Buffer is a lysis buffer specifically designed for exosome samples, capable of efficiently lysing exosome proteins.
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Storage :
-20°C，1 year.
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Description & Advantages
MCE Exosome Protein Lysis Buffer is a lysis buffer specifically designed for exosome samples, capable of efficiently lysing exosome proteins. The lysed protein samples can be used in experiments such as BCA, SDS-PAGE, and Western Blot.
This product contains various inhibitors including sodium deoxycholate, EDTA, PMSF, and a Protease Inhibitor Cocktail (AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A), which effectively prevent protein degradation.
Protocol
1. Experimental Preparation
Before the experiment, thaw the Exosome Protein Lysis Buffer at 4°C. Once thawed, take an appropriate amount and keep it on ice for use.
Note: It is recommended to aliquot the lysis buffer after the first thaw to avoid repeated freeze-thaw cycles.
2. Exosome Lysis
Mix the exosome sample with the lysis buffer at a 1:1 volume ratio, and incubate on ice for 10 min.
3. Protein Collection
Centrifuge at 4°C, 11,200 g (12,000 rpm) for 5 min, and collect the supernatant. The protein concentration in the supernatant can be quantified using the BCA method, and then used for Western Blot and other subsequent experiments.
Note: a. The above centrifugation is based on a small centrifuge (effective radius ~ 7 cm) and ≤ 2 mL centrifuge tubes. For larger volumes, it is recommended to centrifuge in aliquots.
b. The protein concentration after lysis should be controlled below 2 mg/mL to ensure complete lysis.
Publications
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Journal Impact Factor
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Most Recent
Storage
-20°C，1 year.
Attention
1. All steps of protein extraction must be performed on ice or at 4°C to maintain protein activity.
2. Due to the high detergent concentration, the Bradford assays cannot be used to measure protein concentration in samples obtained using this lysis buffer.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
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