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  5. G418 Selective Antibiotic, Sterile

G418 Selective Antibiotic, Sterile 

Cat. No.: HY-K1056
Manual SDS

MCE G418 Selective Antibiotic, Sterile is an aminoglycoside antibiotic produced by Micromonospora rhodorangea. It acts by binding to the 80S subunit of the bacterial ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.

G418 Selective Antibiotic, Sterile
Size Price Stock Quantity
Free Sample (1 mL)   Apply Now  
10 mL USD 62 In-stock
Estimated Time of Arrival: December 31
50 mL USD 215 In-stock
Estimated Time of Arrival: December 31

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

G418 selective antibiotic is an aminoglycoside antibiotic produced by Micromonospora rhodorangea. It acts by binding to the 80S subunit of the bacterial ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.

Resistance to G418 selective antibiotic is conferred by the E. coli APH (3) I and APH (3) II resistance genes. The gene becomes APH (3) II on Tn5 and APH (3) I on Tn601. Therefore, G-418 selective antibiotic can be used to select and maintain prokaryotic or eukaryotic cells carrying the G418 resistance gene.

Storage

-20°C; 2 years.

Protocol

1.   The working concentration of G418 selective antibiotic varies with cell type, media, growth conditions and cell metabolic rate.

选择性抗生素
Cell Transposon Gene Concentration (mg/L) Remark
Slime mold Tn5 APH (3) II
10
35
Cells grow in culture
Cells attached to
freeze-dried bacteria
Mammalian Tn5 APH (3) II
400
200
Used to filter
Used to maintain
Plant Tn5 APH (3) II 10 Used to filter
Yeast Tn601 APH (3) I
500
125
Used to filter
Used to maintain

1.   Before stable transfected cell lines can be selected, the optimal G418 selective antibiotic concentration needs to be determined by performing a kill curve titration.

   1)    Seed the parental cell line in a suitable culture plate at a cell density of 20-25% and incubate the cells for 24 hours at 37°C.

   2)   Remove medium and then add medium with various concentrations of G418 selective antibiotic (such as 0, 50, 100, 200, 400, 800, and 1000 μg/mL) and incubate at 37°C.

   3)   Refresh the selective medium every 2-3 days and observe the percentage of surviving cells over time.

   4)   Determine the lowest concentration of antibiotic that kills a large majority of the cells within 14 days. This concentration should be used for selection of a stable transfected cell line.

Components
Components HY-K1056-10 mL HY-K1056-50 mL
G418 Selective Antibiotic, Sterile (50 mg/mL) 10 mL 50 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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G418 Selective Antibiotic, Sterile
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