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Cat. No.: HY-K0234

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Glutathione Magnetic Agarose Beads

Name: Glutathione Magnetic Agarose Beads
Brand: MedChemExpress
SKU: HY-K0234
Price: 175.00 USD
Availability: InStock
Rating: 5 (2 reviews)

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

MCE Glutathione Magnetic Agarose Beads have high protein-binding capacity and stability, making it ideal for high performance purification of GST-tagged fusion proteins expressed in E. coli, yeast, insect and mammalian expression systems.

Storage :
4°C, 2 years. Do not dry or freeze.

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Description & Advantages

MCE Glutathione Magnetic Agarose Beads are based on magnetic cross-linked agarose, effectively purify high levels of overexpressed GST-tagged fusion proteins at a variety of scales. MCE Glutathione Magnetic Agarose Beads have high protein-binding capacity and stability, making it ideal for high performance purification of GST-tagged fusion proteins expressed in E. coli, yeast, insect and mammalian expression systems.

• Convenient and time saving.

• Low non-specific binding.

• Minimal sample loss.

• Protein binding capacity up to 5-10 mg/mL Settled Beads.

• Stable, one bottle solution.

The specifications of the product correspond to the actual beads volume, with the beads content of 20%.

Protocol

Recommended Buffers (Not Provided)

Buffer	Composition
Binding/Wash Buffer	1× PBS, pH 7.4; or 50 mM Tris-HCl, 150 mM NaCl, pH 7.4
Elution Buffer	50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
Storage Buffer	1× PBS containing 20% ethanol

Note: Prepare all buffers with ultrapure water and filter them through a 0.22 μm or 0.45 μm membrane.

Sample Preparation

Lyse samples containing GST-tagged proteins from bacterial, yeast, insect, or mammalian expression systems under cold conditions and clarify them by centrifugation. Filter through a 0.22 μm or 0.45 μm membrane before loading. Dialyze or dilute samples containing high concentrations of detergents or denaturants.

GST-Tagged Protein Purification

1. Bead Pretreatment: Thoroughly resuspend the Glutathione Magnetic Agarose Beads, transfer the required volume into a tube, perform magnetic separation, discard the storage solution, and wash with 5 bead volumes of binding/wash buffer 2-3 times.

2. Sample Binding: Add the clarified GST-tagged protein sample and incubate on a rotator at 4°C for 1-2 h or overnight. Perform magnetic separation and retain the flow-through for analysis if required.

3. Washing: Wash with 5-10 bead volumes of binding/wash buffer 3-5 times.

4. Elution: Add 3-5 bead volumes of elution buffer, incubate at room temperature for 5-10 min, perform magnetic separation, and collect the supernatant. Repeat 2-3 times if necessary. For SDS-PAGE or Western blotting only, add SDS-PAGE Loading Buffer, heat at 95°C for 5 min, perform magnetic separation, and collect the supernatant.

5. SDS-PAGE Analysis: Analyze the original sample, flow-through, wash, and elution fractions to assess purification.

6. Storage: If the beads will be reused, wash thoroughly with binding/wash buffer and ddH₂O, resuspend in storage buffer, and store at 2-8°C. Do not freeze, dry, or expose the beads to a magnetic field for an extended period.