L-Glutathione reduced (Synonyms: GSH; γ-L-Glutamyl-L-cysteinyl-glycine)

Name: L-Glutathione reduced
Brand: MedChemExpress
SKU: HY-D0187
Rating: 5.0 (54 reviews)

Cat. No.: HY-D0187 Purity: 99.79%: Data Sheet
COA

SDS
Handling Instructions
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L-Glutathione reduced (GSH; γ-L-Glutamyl-L-cysteinyl-glycine) is an endogenous antioxidant and is capable of scavenging oxygen-derived free radicals.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 70-18-8

Size	Stock	Quantity
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Based on 54 publication(s) in Google Scholar

Other Forms of L-Glutathione reduced:

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54 Publications Citing Use of MCE L-Glutathione reduced

RT-PCR

Cell Proliferation/Viability Assay

: L-Glutathione reduced purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 22;16(1):3777. [Abstract]; RT‒qPCR analyses demonstrated that the transcriptional upregulation of sHSP genes (17.4, 17.6A, 21, 23.5) in ClpD-GFP line #5 was suppressed by the ROS scavenging reagent GSH (500 µM, 2 days). HSP18.5 and the transcripts encoding two large HSPs (HSP90C and mtHSC70-1) were used as negative controls.

: L-Glutathione reduced purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul:84:103693. [Abstract]; BR-CHAC1-OE and MR-CHAC1-OE cells were treated with DMSO, AZD4547 (2.5 μM) with or without GSH (10 mM) and NAC (5 mM) for 72 h, and cell viability was measured by the CCK-8 assay.

: L-Glutathione reduced purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Aug:74:589-607. [Abstract]; Cell viability was measured after being pretreated with Fer-1 (2 μM), GSH (1 mM), or DFO (50 μM) for 1 h, and then treated with or without Sal (50 μM) for 24 h (n = 6).

: L-Glutathione reduced purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Dec;11(12):4045-4054.; KU-19-19 was treated with baicalin with or without glutathione (GSH) (1.5 mM) for 24 h, then cell viability was analyzed.

: L-Glutathione reduced purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2020 May 8;5(1):51. [Abstract]; H460 and H1299 cells were treated with erianin with or without the ROS scavenger GSH (1 mM) for 24 h, and cell viability was assayed.

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Human Endogenous Metabolite Microbial Metabolite

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Purity & Documentation
References
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Description

L-Glutathione reduced (GSH; γ-L-Glutamyl-L-cysteinyl-glycine) is an endogenous antioxidant and is capable of scavenging oxygen-derived free radicals.

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
KB 3-1	IC₅₀	> 20 mM Compound: GSH	Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay	[PMID: 21657271]
KB-V1	IC₅₀	> 20 mM Compound: GSH	Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay	[PMID: 21657271]

In Vitro

L-Glutathione reduced is a non-protein thiol widely exists in living cells. L-Glutathione reduced plays important biological functions in the organism, including protein and DNA synthesis, enzyme activity, metabolism and cell protection. L-Glutathione reduced is capable of scavenging oxygen-derived free radicals and is established to be a marker of oxidative stress^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

307.32

Formula

C₁₀H₁₇N₃O₆S

CAS No.

70-18-8

Appearance

Solid

Color

White to off-white

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : 62.5 mg/mL (203.37 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2539 mL	16.2697 mL	32.5394 mL
5 mM	0.6508 mL	3.2539 mL	6.5079 mL
10 mM	0.3254 mL	1.6270 mL	3.2539 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (325.39 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (271 KB) SDS (394 KB)

COA (244 KB) HNMR (254 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Pereira-Rodrigues N, et al. Electrocatalytic activity of cobalt phthalocyanine CoPc adsorbed on a graphite electrode for the oxidation of reduced L-glutathione (GSH) and the reduction of its disulfide (GSSG) at physiological pH. Bioelectrochemistry. 2007 Jan;70(1):147-54. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.2539 mL	16.2697 mL	32.5394 mL	81.3484 mL
	5 mM	0.6508 mL	3.2539 mL	6.5079 mL	16.2697 mL
	10 mM	0.3254 mL	1.6270 mL	3.2539 mL	8.1348 mL
	15 mM	0.2169 mL	1.0846 mL	2.1693 mL	5.4232 mL
	20 mM	0.1627 mL	0.8135 mL	1.6270 mL	4.0674 mL
	25 mM	0.1302 mL	0.6508 mL	1.3016 mL	3.2539 mL
	30 mM	0.1085 mL	0.5423 mL	1.0846 mL	2.7116 mL
	40 mM	0.0813 mL	0.4067 mL	0.8135 mL	2.0337 mL
	50 mM	0.0651 mL	0.3254 mL	0.6508 mL	1.6270 mL
	60 mM	0.0542 mL	0.2712 mL	0.5423 mL	1.3558 mL
	80 mM	0.0407 mL	0.2034 mL	0.4067 mL	1.0169 mL
	100 mM	0.0325 mL	0.1627 mL	0.3254 mL	0.8135 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-Glutathione reduced Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Glutathione reduced70-18-8GSH γ-L-Glutamyl-L-cysteinyl-glycineEndogenous MetaboliteReactive Oxygen Species (ROS)FerroptosisInhibitorinhibitorinhibit

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L-Glutathione reduced (Synonyms: GSH; γ-L-Glutamyl-L-cysteinyl-glycine)

Customer Review

Other Forms of L-Glutathione reduced:

L-Glutathione reduced Related Antibodies

54 Publications Citing Use of MCE L-Glutathione reduced

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

L-Glutathione reduced Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

L-Glutathione reduced Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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