Cat. No.: HY-Y0337

L-Cysteine

(Synonyms: Cysteine)

12 Cited Publications

Customer Review

Based on 12 publication(s) in Google Scholar

L-Cysteine (Cysteine) is an orally active conditionally essential amino acid with hypoglycemic effects, which acts as a precursor for biologically active molecules such as hydrogen sulphide (H2S), glutathione and taurine. L-Cysteine promotes the proliferation and differentiation of neural stem cells via the CBS/H2S pathway. L-Cysteine suppresses ghrelin and reduces appetite in rodents and humans. L-Cysteine can be used as an anorectic agent.

For research use only. We do not sell to patients.

Purity: 99.3%
CAS No.: 52-90-4
Formula: C₃H₇NO₂S
Molecular Weight:121.16

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

L-Cysteine Chemical Structure

CAS No.: 52-90-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (12)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

ERK1

ERK2

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	161 μM Compound: 5862	Substrate uptake by the Excitatory Amino Acid Transporter 3 (EAAT3, SLC1A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A1 cells Substrate uptake by the Excitatory Amino Acid Transporter 3 (EAAT3, SLC1A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A1 cells	10.5281/zenodo.7361326
HEK293	EC₅₀	161 μM Compound: 5862	Substrate uptake by the Excitatory Amino Acid Transporter 3 (EAAT3, SLC1A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A1 cells (PubChem AID: 1794812) Substrate uptake by the Excitatory Amino Acid Transporter 3 (EAAT3, SLC1A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A1 cells (PubChem AID: 1794812)	10.5281/zenodo.7361326
HEK293	EC₅₀	376 μM Compound: 5862	Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells	10.5281/zenodo.6782621
HEK293	EC₅₀	376 μM Compound: 5862	Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745864) Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745864)	10.5281/zenodo.6782621
HeLa	IC₅₀	>50 μM Compound: 3a	Cytotoxic activity against HeLa cells Cytotoxic activity against HeLa cells	[PMID: 17524640]
SNB-19	IC₅₀	116 μM Compound: L-cystine	Inhibition of amino acid transport system xc- in human SNB19 cells assessed as [3H]L-glutamate uptake by liquid scintillation counting Inhibition of amino acid transport system xc- in human SNB19 cells assessed as [3H]L-glutamate uptake by liquid scintillation counting	[PMID: 19932968]

In Vitro

L-Cysteine (0.1-50 μM, 24 and 48 h) increases cell viability of neural stem cells (NSCs) and promotes proliferation and neuronal differentiation of NSCs^[2].
L-cysteine (1 μM, 0-90 min) activates ERK1/2 via CBS/H2S pathway in NSCs^[2].
L-Cysteine (0.1-20 mM, 30 min) results in a decrease in glucose uptake in soleus muscle^[3].
L-Cysteine (0.5 mM, 30 min) increases GLUT3 levels in SH-SY5Y cells and GLUT4 levels in SH-SY5Y cells, soleus muscle and C2C12 muscle cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

NSCs
Concentration:

0.1-50 μM
Incubation Time:

24 and 48 h
Result:

Obviously improved the cell viability of NSCs in a dose-dependent manner at lower doses (0.5, 1, 5 μM) and increased the ratio of BrdU-positive cells against total NSCs at 24 h.

Cell Viability Assay^[2]

Cell Line:

NSCs
Concentration:

1 μM
Incubation Time:

0-90 min
Result:

Induced overexpression of phosphorylated ERK (p-ERK1/2) in NSCs.

In Vivo

L-Cysteine (1-4 mmol/kg, p.o. or i.p., a single dose for 1 h) decreases food intake in rodents, increases respiratory exchange ratio (RER) in mice, increases neuronal activation in the rat brainstem, reduces gastric emptying and suppresses plasma acyl ghrelin levels in rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wistar rats following an overnight 16-h fast^[1]
Dosage:

1-4 mmol/kg
Administration:

p.o. or i.p., a single dose for 1 h
Result:

Dose-dependently decreased food intake in rats and mice and significantly increased cFLI in the area postrema in rats.

Chemical Information

CAS No. 52-90-4
Appearance Solid
Molecular Weight 121.16
Formula C₃H₇NO₂S
Color White to off-white
SMILES

N[C@@H](CS)C(O)=O
Synonyms

Cysteine
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (12)

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Cell Metab

Lighting up arginine metabolism reveals its functional diversity in physiology and pathology. [Abstract]

2025 Jan 7;37(1):291-304.e9. PMID: 39413790
Adv Sci (Weinh)

Targeting the SOX9/TIMP1 Axis with iRGD-Conjugated Nanoplatform Enhances Dendritic Cell Function and Photodynamic Immunotherapy in Gastric Cancer. [Abstract]

2025 Nov 21:e10500. PMID: 41270217
J Nanobiotechnology

Targeted inhibition of RGS19 alleviates renal fibrosis by restoring autophagy and modulating immune cell infiltration. [Abstract]

2026 Feb 28;24(1):315. PMID: 41761314
Int J Biol Sci

MKL-1 suppresses ferroptosis by activating system Xc- and increasing glutathione synthesis. [Abstract]

2023 Aug 21;19(14):4457-4475. PMID: 37781038
Sci Total Environ

Network pharmacology and transcriptomics reveal androgen receptor as a potential protein target for 6PPD-quinone. [Abstract]

2024 Nov 27:957:177678. PMID: 39581451
Probiotics Antimicrob Proteins

Potential Benefits of Metformin Via Roseburia Intestinalis in High-fat Diet-fed Mice With Colorectal Adenomas. [Abstract]

2025 Nov 26. PMID: 41296177
J Chromatogr A

Preparation of thiol-free amino acid plasma matrix for quantification of thiol amino acids and their oxidized forms in human plasma by UPLC-MS/MS. [Abstract]

2024 Apr 12:1720:464776. PMID: 38432109
Life Metab

2025 Oct 8.
Amino Acids

Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]

2024 Sep 18;56(1):56. PMID: 39292313
Research Square Preprint

2024 Jan 24.
SSRN

2023 Nov 17.

Solvent & Solubility

In Vitro:

H₂O : 5 mg/mL (41.27 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

8.2535 mL

41.2677 mL

82.5355 mL

5 mM

1.6507 mL

8.2535 mL

16.5071 mL

10 mM

0.8254 mL

4.1268 mL

8.2535 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (825.35 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. McGavigan AK, et al. L-cysteine suppresses ghrelin and reduces appetite in rodents and humans. Int J Obes (Lond). 2015 Mar;39(3):447-55. [Content Brief]

[2]. Wang Z, et al. L-Cysteine promotes the proliferation and differentiation of neural stem cells via the CBS/H₂S pathway. Neuroscience. 2013 May 1;237:106-17. [Content Brief]

[3]. Gazit V, et al. L-cysteine increases glucose uptake in mouse soleus muscle and SH-SY5Y cells. Metab Brain Dis. 2003 Sep;18(3):221-31. [Content Brief]

[4]. Takemoto Y. The central effect of L-cysteine on cardiovascular system of the conscious rat. Jpn J Physiol. 1995;45(5):771-83. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	8.2535 mL	41.2677 mL	82.5355 mL	206.3387 mL
	5 mM	1.6507 mL	8.2535 mL	16.5071 mL	41.2677 mL
	10 mM	0.8254 mL	4.1268 mL	8.2535 mL	20.6339 mL
	15 mM	0.5502 mL	2.7512 mL	5.5024 mL	13.7559 mL
	20 mM	0.4127 mL	2.0634 mL	4.1268 mL	10.3169 mL
	25 mM	0.3301 mL	1.6507 mL	3.3014 mL	8.2535 mL
	30 mM	0.2751 mL	1.3756 mL	2.7512 mL	6.8780 mL
	40 mM	0.2063 mL	1.0317 mL	2.0634 mL	5.1585 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

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L-Cysteine

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Publications Citing Use of MedChemExpress (MCE) L-Cysteine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (12)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

L-Cysteine Related Classifications

Cat. No.: HY-Y0337