Cat. No.: HY-W016715

L-Cysteine hydrochloride hydrate

12 Cited Publications

Customer Review

Based on 12 publication(s) in Google Scholar

L-Cysteine hydrochloride hydrate is an orally active and essential amino acid, which acts as a precursor for biologically active molecules such as hydrogen sulphide (H2S), glutathione and taurine. L-Cysteine hydrochloride hydrate regulates CBS/H2S pathway, inhibits NF-κB activation and insulin and ghrelin secretion. L-Cysteine hydrochloride hydrate reduces blood sugar, vascular inflammation markers and appetite. L-Cysteine hydrochloride hydrate induces kidney damage. L-Cysteine hydrochloride hydrate can be used in the study of neurological diseases and diabetes.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 7048-04-6
Formula: C₃H₁₀ClNO₃S
Molecular Weight:175.63

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

L-Cysteine hydrochloride hydrate Chemical Structure

CAS No.: 7048-04-6

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (12)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All NF-κB Isoforms :

In Vitro

L-Cysteine hydrochloride (5 mM, 5-7 days) hydrate inhibits Aspergillus flavus growth and AFB1 synthesis by disrupting cell structure and antioxidant system balance^[2].
L-Cysteine (1-10 μM, 1-5 days) hydrochloride hydrate promotes the proliferation and differentiation of neural stem cells via the CBS/H2S pathway^[3].
L-Cysteine (3 mM, 1 h) hydrochloride hydrate inhibits insulin release from the pancreatic β-Cell^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[3]

Cell Line:

neural stem cells
Concentration:

0.1, 1 and 10 μM
Incubation Time:

3, 5 and 7 d
Result:

Improved MAP-2 expression at day 5.
Increased the percentage of MAP-2-positive cells in NSCs (1 μM).
Increased the absolute number of newborn neurons.

In Vivo

L-Cysteine (500-2000 mg/kg/day, p.o., daily, 28 consecutive days) hydrochloride hydrate exhibits renal injuries such as basophilic tubules with eosinophilic material in the lumen in rats^[5].
L-Cysteine (50 mg/kg, i.p., 7 days) hydrochloride hydrate only marginally alleviates lead-induced toxicity in albino mice^[6].
L-Cysteine (1-4 mmol/kg, p.o.) hydrochloride hydrate suppresses ghrelin and reduces appetite in rodents (rats and mice)^[7].
L-Cysteine (1 mg/kg, p.o., daily, 8 weeks) hydrochloride hydrate can lower glycemia and markers of vascular inflammation in diabetes apparently by preventing NF-κB activation in a diabetic rat model^[8].
L-Cysteine (1000 mg/kg, i.v., daily) hydrochloride hydrate suppresses body weight gain, decreases spontaneous activity, salivation, ptosis, tremor in male Sprague-Dawley rats^[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Zucker diabetic rats^[8]
Dosage:

1 mg/kg
Administration:

Oral gavage (p.o.), daily, 8 weeks
Result:

Showed lower glucose levels.
Reduced blood glucose, glycated hemoglobin, CRP, MCP-1.
Decreased levels of activated NF-κB and phosphorylation of Akt.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 7048-04-6
Appearance Solid
Molecular Weight 175.63
Formula C₃H₁₀ClNO₃S
Color White to off-white
SMILES

N[C@@H](CS)C(O)=O.[H]Cl.[H]O[H]
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (12)

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Cell Metab

Lighting up arginine metabolism reveals its functional diversity in physiology and pathology. [Abstract]

2025 Jan 7;37(1):291-304.e9. PMID: 39413790
J Nanobiotechnology

Targeted inhibition of RGS19 alleviates renal fibrosis by restoring autophagy and modulating immune cell infiltration. [Abstract]

2026 Feb 28;24(1):315. PMID: 41761314
Adv Sci (Weinh)

Targeting the SOX9/TIMP1 Axis with iRGD-Conjugated Nanoplatform Enhances Dendritic Cell Function and Photodynamic Immunotherapy in Gastric Cancer. [Abstract]

2025 Nov 21:e10500. PMID: 41270217
Int J Biol Sci

MKL-1 suppresses ferroptosis by activating system Xc- and increasing glutathione synthesis. [Abstract]

2023 Aug 21;19(14):4457-4475. PMID: 37781038
Life Metab

2025 Oct 8.
Probiotics Antimicrob Proteins

Potential Benefits of Metformin Via Roseburia Intestinalis in High-fat Diet-fed Mice With Colorectal Adenomas. [Abstract]

2025 Nov 26. PMID: 41296177
J Chromatogr A

Preparation of thiol-free amino acid plasma matrix for quantification of thiol amino acids and their oxidized forms in human plasma by UPLC-MS/MS. [Abstract]

2024 Apr 12:1720:464776. PMID: 38432109
Amino Acids

Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]

2024 Sep 18;56(1):56. PMID: 39292313
Sci Total Environ

Network pharmacology and transcriptomics reveal androgen receptor as a potential protein target for 6PPD-quinone. [Abstract]

2024 Dec 20:957:177678. PMID: 39581451
Research Square Preprint

2024 Jan 24.
SSRN

2023 Nov 17.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (569.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.6938 mL

28.4689 mL

56.9379 mL

5 mM

1.1388 mL

5.6938 mL

11.3876 mL

10 mM

0.5694 mL

2.8469 mL

5.6938 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (14.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. McGavigan AK, et al. L-cysteine suppresses ghrelin and reduces appetite in rodents and humans. Int J Obes (Lond). 2015 Mar;39(3):447-55. [Content Brief]

[2]. Qiu M, et al. L-Cysteine hydrochloride inhibits Aspergillus flavus growth and AFB1 synthesis by disrupting cell structure and antioxidant system balance. J Hazard Mater. 2023 Oct 5;459:132218. [Content Brief]

[3]. Wang Z, et al. L-Cysteine promotes the proliferation and differentiation of neural stem cells via the CBS/H₂S pathway. Neuroscience. 2013 May 1;237:106-17. [Content Brief]

[4]. Kaneko Y, et al. L-cysteine inhibits insulin release from the pancreatic beta-cell: possible involvement of metabolic production of hydrogen sulfide, a novel gasotransmitter. Diabetes. 2006 May;55(5):1391-7. [Content Brief]

[5]. Shibui Y, et al. Comparisons of l-cysteine and d-cysteine toxicity in 4-week repeated-dose toxicity studies of rats receiving daily oral administration. J Toxicol Pathol. 2017 Jul;30(3):217-229. [Content Brief]

[6]. Mahmoud YI, et al. Effects of L-cysteine on lead acetate induced neurotoxicity in albino mice. Biotech Histochem. 2016 Jul;91(5):327-32. [Content Brief]

[7]. McGavigan AK, et al. L-cysteine suppresses ghrelin and reduces appetite in rodents and humans. Int J Obes (Lond). 2015 Mar;39(3):447-55. [Content Brief]

[8]. Jain SK, et al. L-cysteine supplementation lowers blood glucose, glycated hemoglobin, CRP, MCP-1, and oxidative stress and inhibits NF-kappaB activation in the livers of Zucker diabetic rats. Free Radic Biol Med. 2009 Jun 15;46(12):1633-8. [Content Brief]

[9]. Jain S K, et al. L-Cysteine supplementation lowers blood glucose, glycated hemoglobin, CRP, MCP-1, and oxidative stress and inhibits NF-κB activation in the livers of Zucker diabetic rats. Free Radical Biology and Medicine, 2009, 46(12): 1633-1638.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.6938 mL	28.4689 mL	56.9379 mL	142.3447 mL
	5 mM	1.1388 mL	5.6938 mL	11.3876 mL	28.4689 mL
	10 mM	0.5694 mL	2.8469 mL	5.6938 mL	14.2345 mL
	15 mM	0.3796 mL	1.8979 mL	3.7959 mL	9.4896 mL
	20 mM	0.2847 mL	1.4234 mL	2.8469 mL	7.1172 mL
	25 mM	0.2278 mL	1.1388 mL	2.2775 mL	5.6938 mL
	30 mM	0.1898 mL	0.9490 mL	1.8979 mL	4.7448 mL
	40 mM	0.1423 mL	0.7117 mL	1.4234 mL	3.5586 mL
	50 mM	0.1139 mL	0.5694 mL	1.1388 mL	2.8469 mL
	60 mM	0.0949 mL	0.4745 mL	0.9490 mL	2.3724 mL
	80 mM	0.0712 mL	0.3559 mL	0.7117 mL	1.7793 mL
	100 mM	0.0569 mL	0.2847 mL	0.5694 mL	1.4234 mL

Keywords

L-Cysteine hydrochloride hydrate

12 Cited Publications

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L-Cysteine hydrochloride hydrate

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Publications Citing Use of MedChemExpress (MCE) L-Cysteine hydrochloride hydrate

All Endogenous Metabolite Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (12)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs