Exosome Isolation and Purification Kit (from body fluids)
Based on 1 Customer Validation
MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cerebro-spinal fluid, amniotic fluid, milk, saliva, etc, which can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.
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Storage :
Store at room temperature for two years.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Exosomes are small vesicles (30-150 nm) containing RNA and protein that are secreted by various types of cells in culture, and found in abundance in body fluids including blood, saliva, urine, and breast milk. Exosomes are thought to function as intercellular messengers, delivering their cargo of effector or signaling macromolecules between specific cells.
MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cerebro-spinal fluid, amniotic fluid, milk, saliva, etc, which can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc. This product contains sufficient reagents for processing 60 mL of body fluids.
Protocol
1. Pretreatment of body fluids such as cerebrospinal fluid, amniotic fluid, milk, or saliva
1. Keep fresh body-fluid samples on ice. Place thawed frozen samples on ice immediately.
2. Centrifuge at 3,000 × g for 10 min at 4°C to remove cells and large debris.
3. Transfer the supernatant, centrifuge at 10,000 × g for 10 min at 4°C, and collect the clarified supernatant.
2. Exosome enrichment
1. Add 1 mL Exosome Concentration Solution (ECS) per 4 mL clarified supernatant. Thoroughly mix Exosome Concentration Solution (ECS) before use.
2. Vortex for 1 min and incubate at 4°C for at least 2 h. Longer incubation may increase yield, but incubation longer than 24 h is not recommended.
3. Centrifuge at 10,000 × g for 60 min at 4°C and carefully discard the supernatant. The exosome pellet may not be visible.
4. Thoroughly resuspend the exosome pellet in an appropriate amount of pre-cooled 1× PBS.
5. Centrifuge at 12,000 × g for 2 min at 4°C and collect the supernatant as crude exosomes. If debris remains, repeat the 2 min centrifugation.
3. Exosome purification and storage
1. Transfer crude exosomes to an Exosome Purification Filter (EPF), centrifuge at 3,000 × g for 10 min at 4°C, and collect the purified exosome filtrate.
2. Aliquot purified exosomes and store at -80°C. Avoid repeated freeze-thaw cycles. The purified exosomes may be used for EM, NTA, Western Blot, qPCR, and other downstream analyses.
Storage
Store at room temperature for two years.
Components
| Components | HY-K1065-30T |
|---|---|
| Exosome Concentration Solution (ECS) | 15 mL |
| Exosome Purification Filter (EPF) | 30 Tubes |
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