Cell Invasion Basement Membrane Matrix (Ready-to-Use)
MCE Cell Invasion Basement Membrane Matrix (Ready-to-Use) is formulated in high-glucose DMEM and contains 50 μg/mL gentamicin. With an optimized formulation and working concentration, it requires no additional dilution or preparation and can be directly used for Transwell cell invasion assays to establish an in vitro extracellular matrix barrier model for evaluating the invasive capacity and invasion potential of cells, particularly in studies of tumor cell invasion.
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Storage :
-20°C, 1 year. Shipping with dry ice.
- Manual (2836KB)
- COA
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Basement Membrane Matrix is a natural basement membrane preparation extracted from a mouse tumor rich in extracellular matrix (ECM) proteins. It mimics the in vivo cellular microenvironment and provides structural support for cell growth. The matrix contains abundant ECM components, including: Laminin, Collagen IV, Entactin/Nidogen, Heparan Sulfate Proteoglycans (HSPGs). It also contains various naturally occurring growth factors, including: Insulin-like Growth Factor-1 (IGF-1), Transforming Growth Factor-β (TGF-β), Vascular Endothelial Growth Factor (VEGF), Epidermal Growth Factor (EGF), Basic Fibroblast Growth Factor (bFGF).
MCE Cell Invasion Basement Membrane Matrix (Ready-to-Use) is formulated in high-glucose DMEM and contains 50 μg/mL gentamicin. With an optimized formulation and working concentration, it requires no additional dilution or preparation and can be directly used for Transwell cell invasion assays to establish an in vitro extracellular matrix barrier model for evaluating the invasive capacity and invasion potential of cells, particularly in studies of tumor cell invasion.
The convenient ready-to-use format allows immediate use after opening, minimizing hands-on preparation, streamlining workflow, improving experimental efficiency, and ensuring excellent batch-to-batch consistency and reproducibility.
Protocol
1. Matrigel Thawing: Remove the Matrigel from storage, place it on ice and allow it to thaw slowly in a 4 °C refrigerator until ready for use. Avoid repeated freeze‑thaw cycles during thawing.
2. Matrigel Coating: Add an appropriate volume of Matrigel to the cell culture plate to evenly cover the entire culture surface. Apply the solution slowly and gently swirl the plate to spread the Matrigel uniformly, avoiding the formation of air bubbles. Refer to the table below for recommended volumes for different culture vessels.
| Transwell Plate Format | Insert Outer Diameter (mm) | Polycarbonate Membrane Surface Area (cm²) | Matrigel Volume |
|---|---|---|---|
| 6‑well plate | ~ 24.5 | 4.67 | 1 mL |
| 12‑well plate | ~ 12 | 1.12 | 250 μL |
| 24‑well plate | ~ 6.5 | 0.33 | 60 μL |
3. Matrigel Gelation: Incubate the coated plate in a 37 °C cell culture incubator for ≥ 2 h until the Matrigel is fully polymerized and ready for use.
4. Cell Seeding: Resuspend cells in serum‑free medium, and slowly add the cell suspension to the upper insert along the side wall. Add serum‑containing medium to the lower chamber as a chemoattractant.
Note:
a. Determine the optimal seeding density via preliminary experiments according to cell type and invasive capacity.
b. Handle carefully during seeding to avoid air bubbles between the lower chamber medium and the insert. If bubbles form — which may impair chemotaxis — gently lift the insert to remove the bubbles before placing it back into the plate.
5. Incubation and Result Analysis: Carefully transfer the plate to a cell culture incubator for further incubation. After the designated incubation period, harvest the samples for fixation, staining and result analysis. Observation of cell invasion is recommended after 24 h of culture.
Note:
a. Inspect the plate 1–2 h after seeding to confirm there are no visible air bubbles between the insert and the lower chamber medium.
b. Incubation time may be adjusted according to the invasive capacity of the cells; observation time points of 24–48 h are generally recommended. Comprehensive evaluation should also take cell status and digestion time into account to avoid artifacts caused by changes in cell condition.
Storage
-20°C, 1 year.
Shipping with dry ice.
Attention
1. This product has been optimized for ready-to-use applications. Further dilution is not recommended, as it may affect coating performance.
2. Please bury this product with packaging in ice and thaw in the 4°C refrigerator. After thawing, make aliquots and keep them frozen.
3. The matrix readily gels above 10°C; maintain low temperature throughout handling.
4. Pre-cool all consumables (pipette tips, tubes, culture plates, etc.) before use.
5. Avoid holding the lower portion of containers containing matrix to prevent localized warming and gelation.
6. If the matrix becomes viscous or partially gelled due to warming, place it on ice and incubate at 4°C for 1-2 h until fluidity is restored.
7. During thawing, ensure that the storage container remains tightly sealed to prevent leakage or tube rupture.
8. This product is for R&D use only, not for drug, household, or other uses.
9. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K6025-5 mL | HY-K6025-10 mL |
|---|---|---|
| Cell Invasion Basement Membrane Matrix (Ready-to-Use) | 5 mL | 10 mL |
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