2. Enzymes
  3. Ammonium Sulfate Suspension

Ammonium Sulfate Suspension

Enzymes provided in the form of ammonium sulfate suspension refer to enzyme preparations obtained through precipitation by the ammonium sulfate salt fractionation method. Essentially, the enzyme molecules are suspended as solid particles in the ammonium sulfate solution. Therefore, most of the enzymes in the ammonium sulfate suspension are in solid form, and the amount of enzyme in the solution can be considered negligible.
Preparation before enzyme usage:
1. Take the enzyme out of the refrigerator (Note: The ammonium sulfate suspension must not be frozen). Tighten the cap and invert the vial for about 1 minute to create a milky suspension. Avoid vortexing or sonication during this process to prevent enzyme denaturation.
2. Handle the suspension according to different situations:
1) If ammonium sulfate may interfere with the reaction: Use a pipette with a sterile wide-bore tip to take a portion of the suspension and transfer it to a clean centrifuge tube. Perform a brief low-speed centrifugation at 2℃ - 8℃ to precipitate the enzyme. Carefully remove as much of the supernatant as possible and retain it. It is not necessary to remove all of the supernatant. Add an appropriate amount of reaction buffer to dissolve the precipitate. Measure the protein content and enzyme activity in both the supernatant and the precipitate dissolution solution.
2) If ammonium sulfate does not interfere with the reaction: After taking out the enzyme suspension with a pipette, directly add the suspension to the reaction buffer.

Ammonium Sulfate Suspension (4):

Cat. No. Product Name CAS No.  
  • HY-P2726
    Aldolase, Rabbit muscle 9024-52-6  
    Aldolase, Rabbit muscle (Fructose-diphosphate aldolase) is a glycolytic enzyme and glucose availability sensor. Aldolase, Rabbit muscle catalyzes the reversible carbon-carbon cleavage of fructose-1,6-bisphosphate to produce dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, Rabbit muscle is an essential component for v-ATPase activity. Aldolase, Rabbit muscle participates in processes such as gluconeogenesis, the Calvin cycle and glycolysis.
  • HY-P2765
    Glycerol-3-phosphate dehydrogenase, rabbit muscle 9075-65-4  
    Glycerol-3-phosphate dehydrogenase, rabbit muscle (GPDH) is an enzyme that catalyzes the reversible redox conversion of dihydroxyacetone phosphate to sn-glycerol 3-phosphate. Glycerol-3-phosphate dehydrogenase is a glycolytic enzyme and is responsible for the re-oxidation of NADH to NAD+ and energy supply.
  • HY-E70103
    α-L-Arabinofuranosidase 9067-74-7  
    α-L-Arabinofuranosidase is a kind of glycoside hydrolase. It hydrolyzes the non-reducing ends of α-L-arabinofuranosyl residues to form the side chains of arabinoxylan, arabinogalactan and arabinan.
  • HY-E70104
    α-Rhamnosidase 37288-35-0  
    α-Rhamnosidase is a glycoside hydrolase. α-Rhamnosidase is able to finish the enzymatic de-glycosylation of many nature flavone glycosides.