Halo Green Kit
The kit uses the Halo-tag principle to specifically label living cell structures.
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Storage :
-20°C, 1 year
Keep away from light
- Manual (124KB)
- COA
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Halo Green is a fluorescent probe with green fluorescence that can specifically label various subcellular structures in living mammalian cells, with strong water solubility and pH stability; its maximum excitation wavelength is 490 nm and its maximum emission wavelength is 517 nm. The MCE Halo Green kit adopts the Halo-tag labeling principle, which allows the flexible replacement of recombinant plasmids with a variety of Halo motifs for specific labeling of living cellular structures.
Protocol
1. Prepare the cells.
2. Prepare the working solution for incubation.
3. Wash the cells growing in the well plate or confocal dish with appropriate amount of PBS, and then take appropriate amount of serum-free medium to rinse the cell surface once.
4. Add the working solution and incubate the cells with the working solution for 1 h.
5. Take the appropriate amount of PBS and wash 3 times, add the appropriate amount of serum-free medium and leave for 15 min.
6. Take appropriate amount of PBS and wash 3 times, replace with serum cell culture medium and leave for 15 min.
7. Imaging.
Storage
-20°C, 1 year
Keep away from light
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