Human Airway Organoid Kit 

MCE Human Airway Organoid Kit includes a basic culture medium for Human Airway organoids, as well as culture supplements, and is designed for the effective construction of human airway organoids (hAOs) from human airway stem cells.

The self-renewal of the airway epithelium is driven by the proliferation of basal cells. Human airway organoids comprise basal cells, ciliated cells, secretory cells, and a small number of neuroendocrine cells. As such, these organoids recapitulate all key features of the airway epithelium, including its architecture, cellular composition, and self-renewal dynamics. Therefore, they hold great promise for advancing studies on airway homeostasis and related diseases.

Basal Medium A: 4°C, 1 year. Shipping with blue ice.

Supplement B (50×) & Supplement C (250×): -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry a ice.

1. Cells need to be kept sterile when extracted from primary tissue to avoid contamination from subsequent experiments.

2. Observe the fragmentation status of the organoid during passaged dissociation, and terminate the dissociation when small cell clusters (10-50 cells) appear to avoid prolonging the subsequent growth viability of the organoid.

3. Operations involving MCE Basement Membrane Matrix need to be kept at low temperature throughout. MCE Basement Membrane Matrix should be injected rapidly into the cell culture wells after resuspension with the cells, while avoiding air bubbles.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.