Mitochondria Isolation Kit for Cultured Cells

Cat. No.: HY-K1060: FAQs
Manual SDS

MCE Mitochondria Isolation Kit for Cultured Cells enables the fast and efficient isolation of intact mitochondria from cells using differential centrifugation.

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100T	USD 152	In-stock	Estimated Time of Arrival: December 31

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Description
Storage
Components
Documentation

Description
& Advantages

Mitochondria, the site of most energy production in eukaryotic cells, have a double membrane structure: an outer membrane and a folded inner membrane. The key to the preparation of mitochondria is to ensure the integrity and purity of mitochondria.

MCE Mitochondria Isolation Kit for Cultured Cells enables the fast and efficient isolation of mitochondria from cells using differential centrifugation. Most of the isolated mitochondria have intact inner and outer membranes, as well as physiological functions. In addition, this kit can also be used to extract mitochondrial proteins and mitochondria-free cytoplasmic protein. This kit contains enough reagents for 50-100 isolation procedures from 2-5×107 cells.

Publications

Storage

-20°C, 1 year.

Components

Components	HY-K1060
Mitochondria Isolation Reagent	125 mL
Trypan Blue	10 mL
Mitochondria Storage Buffer	15 mL
Mitochondria Lysis Buffer	15 mL
PMSF (crystal)	For 1.5 mL 100 mM
PMSF (solvent)	1.5 mL

Documentation

Manual (98 KB) SDS (690 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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