1. Immunology/Inflammation
    NF-κB
    Metabolic Enzyme/Protease
  2. Reactive Oxygen Species
  3. H2DCFDA

H2DCFDA (Synonyms: DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate)

Cat. No.: HY-D0940 Purity: 99.82%
Handling Instructions

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS).

For research use only. We do not sell to patients.

H2DCFDA Chemical Structure

H2DCFDA Chemical Structure

CAS No. : 4091-99-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
50 mg USD 60 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 33 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA[1].

Molecular Weight

487.29

Formula

C₂₄H₁₆Cl₂O₇

CAS No.

4091-99-0

SMILES

O=C(O)C1=CC=CC=C1C2C3=C(OC4=C2C=C(Cl)C(OC(C)=O)=C4)C=C(OC(C)=O)C(Cl)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 150 mg/mL (307.82 mM)

Ethanol : 14.29 mg/mL (29.33 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0522 mL 10.2608 mL 20.5217 mL
5 mM 0.4104 mL 2.0522 mL 4.1043 mL
10 mM 0.2052 mL 1.0261 mL 2.0522 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay

ROS Measurements[1]
For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.82%

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Keywords:

H2DCFDADCFH-DA2',7'-Dichlorodihydrofluorescein diacetateReactive Oxygen SpeciesInhibitorinhibitorinhibit

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