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  3. FERONIA receptor kinase-regulated reactive oxygen species mediate self-incompatibility in Brassica rapa

FERONIA receptor kinase-regulated reactive oxygen species mediate self-incompatibility in Brassica rapa

  • Curr Biol. 2021 Jul 26;31(14):3004-3016.e4. doi: 10.1016/j.cub.2021.04.060.
Lili Zhang 1,  Jiabao Huang 2,  Shiqi Su 1,  Xiaochun Wei 3,  Lin Yang 1,  Huanhuan Zhao 1,  Jianqiang Yu 1,  Jie Wang 1,  Jiyun Hui 1,  Shiya Hao 4,  Shanshan Song 1,  Yanyan Cao 1,  Maoshuai Wang 1,  Xiaowei Zhang 3,  Yanyan Zhao 3,  Zhiyong Wang 3,  Weiqing Zeng 5,  Hen-Ming Wu 6,  Yuxiang Yuan 7,  Xiansheng Zhang 8,  Alice Y Cheung 6,  Qiaohong Duan 9
Affiliations

Affiliations

  • 1 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018 Shandong, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018 Shandong, China.
  • 2 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018 Shandong, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018 Shandong, China. Electronic address: [email protected].
  • 3 Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, 450002 Henan, China.
  • 4 School of Arts and Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
  • 5 9220 Enfield Drive, Johnston, IA 50131, USA.
  • 6 Department of Biochemistry and Molecular Biology, Molecular Cell Biology and Plant Biology Programs, University of Massachusetts, Amherst, MA 01003, USA.
  • 7 Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, 450002 Henan, China. Electronic address: [email protected].
  • 8 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018 Shandong, China.
  • 9 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018 Shandong, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018 Shandong, China. Electronic address: [email protected].
PMID: 34015250 DOI: 10.1016/j.cub.2021.04.060
Abstract

Most Plants in the Brassicaceae evolve self-incompatibility (SI) to avoid inbreeding and generate hybrid vigor. Self-pollen is recognized by the S-haplotype-specific interaction of the pollen ligand S-locus protein 11 (SP11) (also known as S-locus cysteine-rich protein [SCR]) and its stigma-specific S-locus receptor kinase (SRK). However, mechanistically much remains unknown about the signaling events that culminate in self-pollen rejection. Here, we show that self-pollen triggers high levels of Reactive Oxygen Species (ROS) in stigma papilla cells to mediate SI in heading Chinese cabbage (Brassica rapa L. ssp. pekinensis). We found that stigmatic ROS increased after self-pollination but decreased after compatible(CP)- pollination. Reducing stigmatic ROS by scavengers or suppressing the expression of respiratory burst oxidase homologs (Rbohs), which encode plant NADPH oxidases that produce ROS, both broke down SI. On the other hand, increasing the level of ROS inhibited the germination and penetration of compatible pollen on the stigma, mimicking an incompatible response. Furthermore, suppressing a B. rapa FERONIA (FER) receptor kinase homolog or Rac/Rop guanosine triphosphatase (GTPase) signaling effectively reduced stigmatic ROS and interfered with SI. Our results suggest that FER-Rac/Rop signaling-regulated, NADPH oxidase-produced ROS is an essential SI response leading to self-pollen rejection.

Keywords

AS-ODN; Chinese cabbage; FERONIA; NADPH oxidases; compatible; ornamental kale; pollen-stigma interactions; radish; reactive oxygen species; self-incompatibility.

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