Exosome Purification Filter
MCE Exosome Purification Filter is designed for use with the corresponding exosome extraction/purification kit and is suitable for the further purification of crude exosome preparations. It helps remove residual impurities and facilitates the preparation of exosome samples with improved purity.
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Storage :
Store at room temperature for 2 years.
- Manual (1382KB)
- COA
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Exosomes are nanoscale extracellular vesicles secreted by cells, typically ranging from 30–150 nm in diameter. They contain RNA, proteins, and other bioactive molecules and are widely present in various biological fluids, including blood, saliva, urine, and breast milk. Exosomes serve as important carriers for intercellular information transfer and participate in cell–cell communication, playing roles in various physiological and pathological processes.
MCE Exosome Purification Filter is designed for use with the corresponding exosome extraction/purification kit and is suitable for the further purification of crude exosome preparations. It helps remove residual impurities and facilitates the preparation of exosome samples with improved purity.
Protocol
1. Exosome Extraction
Use an appropriate exosome extraction kit, such as the Serum/Plasma Exosome Extraction and Purification Kit (MCE Cat. No.: HY-K1063), according to the corresponding instructions to obtain the crude exosome preparation.
2. Exosome Purification
Transfer the collected crude exosome preparation to the upper chamber of the exosome purification column and centrifuge at 3,000 g (~6,200 rpm) for 10 min at 4°C. After centrifugation, collect the liquid at the bottom of the filter tube as the purified exosome preparation.
Note: The above centrifugation conditions are based on a small centrifuge with an effective radius of approximately 7 cm and centrifuge tubes with a capacity of ≤ 2 mL.
3. Exosome Storage
Aliquot the purified exosomes and store at -80°C until use. Avoid repeated freeze-thaw cycles to preserve exosome integrity and associated biological activity.
Storage
Store at room temperature for 2 years.
Attention
1. If the purified exosomes will subsequently be used for co-culture with cells, the exosome preparation may be sterilized by filtration through a 0.22 μm membrane filter.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K1066-20 Tubes |
|---|---|
| Exosome Purification Filter | 20 Tubes |
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