Hematoxylin-Eosin Staining Kit (Including Differentiation and Bluing Solutions) 

Hematoxylin is a natural basic dye extracted from Haematoxylon campechianum that binds to negatively charged acidic substances, such as DNA, staining the chromatin in cell nuclei blue-purple. Eosin is a synthetic acidic dye that binds to positively charged amino cations in proteins, staining the cytoplasm and extracellular matrix red or pink. After staining with Hematoxylin and Eosin, the cell nuclei appear blue or blue-purple, while the cytoplasm and extracellular matrix are stained red or pink. This significantly enhances the contrast of tissue structures, making histological studies more intuitive.

MCE Hematoxylin-Eosin Staining Kit (Including Differentiation and Bluing Solutions) contains staining solutions, differentiation solution, and bluing solution. Through optimized formulations, it helps to adjust the staining effect, ensuring proper staining intensity and further improving experimental accuracy.

This kit is suitable for staining tissue paraffin sections, frozen sections, and cultured cells in immunohistochemistry, particularly for histological and pathological studies where precise observation of cell nuclei and cytoplasm structures is required.

Room temperature, 1 year.

Protected from light.

1. Differentiation after staining helps to clarify the nuclear-cytoplasmic staining, but the time should not be too long. The differentiation time with ethanol can be adjusted based on the section thickness, tissue type, and the freshness of the differentiation solution. The rinse after differentiation is to thoroughly remove the acid.

2. Staining solution can be reused; replace with fresh staining solution when staining performance significantly decreases.

3. Since ethanol is highly volatile, graded ethanol solutions should be freshly prepared before use to ensure consistent dehydration performance.

4. Hematoxylin staining may not perform well at lower temperatures; it is advisable to extend the staining time appropriately.

5. At higher room temperatures, the deparaffinization time can be reduced slightly.

6. Bluing solution can also be replaced with 0.2-1% ammonia solution, Scott’s bluing solution, or 0.1-1% lithium carbonate solution.

7. After staining with this kit, subsequent immunofluorescence or other fluorescence-based staining is not recommended, as it may interfere with fluorescence signals or the interpretation of staining results.

8. This product is for R&D use only, not for drug, household, or other uses.

9. For your safety and health, please wear a lab coat and disposable gloves to operate.