2. Enzymes
  3. Protease

Protease

Proteases, usually divided into serine proteases, cysteine proteases, metalloproteases and aspartic proteases, are widely found in animal organs, plant stems and leaves, fruits and microorganisms.

Protease are mainly used for:

• Catalyzing the hydrolysis of proteins and peptides

• Used in protein cleavage experimental procedures

Protease (125):

Cat. No. Product Name CAS No.  
  • HY-P2974
    Elastase, Porcine pancreas 39445-21-1  
    Elastase, Porcine pancreas (EC 3.4.21.36) is a single polypeptide chain of 240 amino acid residues, derived from pig pancreas. Elastase, Porcine pancreas is a serine protease that can hydrolyze proteins and polypeptide. Elastase from porcine pancreas can induce emphysema in hamsters.
  • HY-P1635
    Pepsin 9001-75-6  
    Pepsin is the major pig and human gastric proteases, it is a pepsin-like minor gastric proteolytic enzymes. Pepsin contributes to proteolysis of food proteins in the vertebrate stomach.
  • HY-129047C
    TPCK-treated Trypsin 9002-07-7  
    TPCK-treated Trypsin is a trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone. TPCK-treated Trypsin reduces autolysis and nonspecific proteolysis during experiments, exhibits stability in storage and handling. TPCK-treated trypsin can be used in proteomics research. TPCK-treated Trypsin renders the virus hemagglutinin active, which allows multicycle replication of the virus. TPCK-treated Trypsin can be used for the study of influenza virus.
  • HY-108910
    Chymotrypsin 9004-07-3  
    Chymotrypsin (EC 3.4.21.1; Chymotrypsin A) is an orally effective inhibitor targeting molecules such as TLR4, NF-κB, MMP-1, TNF-α, IL-1β, and IL-6. Chymotrypsin downregulates the TLR4/NF-κB signaling pathway, inhibiting the release of inflammatory factors, reducing cell infiltration and tissue damage. It also reduces the expression of tumor cell adhesion molecules (such as CD44 and CD54) and can be specifically detected by fluorescent probes (such as NBD-3). Chymotrypsin has anti-inflammatory, hepatoprotective, joint damage-reducing, liver protection against lipotoxicity, and anti-tumor metastasis functions. It can be used in research on diseases such as rheumatoid arthritis, non-alcoholic fatty liver disease, and melanoma metastasis. Chymotrypsin can be used in studies of inflammation, edema, and expectoration.
  • HY-P1748
    Thermolysin, Bacillus thermoproteolyticus rokko 9073-78-3  
    Thermolysin, Bacillus thermoproteolyticus rokko (EC 3.4.24.27) (TML) is a thermostable neutral metalloproteinase enzyme secreted by the Gram-positive bacteria Bacillus thermoproteolyticus. Thermolysin catalyzes the hydrolysis of peptide bonds containing hydrophobic residues.

    Optimal pH: 8.0. Considerably stable from pH 5 to 9.5.
    Optimal temperature : 70 °C
  • HY-E71335
    γ-D-Glutamyl-meso-diaminopimelate peptidase  
    γ-D-Glutamyl-meso-diaminopimelate peptidase (EC 3.4.19.11) is a 45-kDa metallopeptidase from Bacillus sphaericus, the substrates being components of the bacterial spore wall. A member of peptidase family M14 (carboxypeptidase A family) . Endopeptidase II has similar activity, but differs in cellular location, molecular mass and catalytic mechanism.
  • HY-E71277
    β-Aspartyl-peptidase  
    β-Aspartyl-peptidase (EC 3.4.19.5) is a mammalian, cytosolic enzyme.
  • HY-E71334
    γ-D-Glutamyl-L-lysine dipeptidyl-peptidase  
    γ-D-Glutamyl-L-lysine dipeptidyl-peptidase (EC 3.4.14.13) , characterized from the bacterium Bacillus subtilis, is involved in the recycling of the murein peptide.
  • HY-129047A
    Trypsin (MS grade) 9002-07-7  
    Trypsin MS grade is a serine protease enzyme, and hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin MS grade activates PAR2 and PAR4. Trypsin MS grade induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin MS grade also promotes cell proliferation and differentiation. Trypsin MS grade can be used in the research of wound healing and neurogenic inflammation.
  • HY-P3208
    Endoproteinase Lys-C 72561-05-8  
    Endoproteinase Lys-C is a protease that cleaves proteins on the C-terminal side of lysine residues and is commonly used for protein sequencing.
  • HY-P3208A
    Endoproteinase Lys-C (Tag-free) 72561-05-8  
    Endoproteinase Lys-C (Tag-free) is a protease that cleaves proteins on the C-terminal side of lysine residues and is commonly used for protein sequencing.
  • HY-E70076
    Subtilisin, bacillus licheniformis 9014-01-1  
    Subtilisin (Compound proteinase) (EC 3.4.21.62) is a proteolytic enzyme, isolated from Bacillus licheniformis. Subtilisin (Compound proteinase) has catalytic activity in anhydrous dimethyl formamide. Subtilisin (Compound proteinase) can be used as a catalyst for easy coupling between sugars and amino acids.
  • HY-E70563
    Endoproteinase Lys-N (MS grade) 91116-93-7  
    Endoproteinase Lys-N (MS grade) is a protease that specifically hydrolyzes the N-terminal peptide bond of lysine fragments.
  • HY-E70370
    hrv 3c Protease  
    hrv 3c Protease is a protease originated from human rhinoviruses. hrv 3c Protease recognizes the sequence LEVLFQGP and cleaves precisely between the Q and GP residues. hrv 3c Protease can be used to remove additional tags from the target proteins.
  • HY-P2953
    Heparitin sulfate lyase 37290-86-1  
    Heparitin sulfate lyase (Heparinase III) is a glycosidic lyase targeting heparan sulfate and heparin. Heparitin sulfate lyase is promising for research of low-molecular-weight heparin production and cancers.
  • HY-P2797A
    Chitinase, Streptomyces griseus 9001-06-3  
    Chitinase, Streptomyces griseus is a chitinase from Streptomyces griseus. Chitinase is a chitin-targeting enzyme with chitin hydrolysis activity. Chitinase inhibits chitin-induced innate type 2 inflammation in the lung. Chitinase augments chitin-free, allergen-induced Th2 inflammation. Chitinase mediates effector functions of IL-13.
  • HY-E70004
    Microbial neutral proteinase 9068-59-1  
    Microbial neutral proteinase is a neutral proteinase from microorganisms and is useful for cell isolation and tissue dissociation.
  • HY-P2729
    Subtilisin 9014-01-1  
    Subtilisin (EC 3.4.21.14) is a bacterial serine protease. Subtilisin induces Apoptosis. Subtilisin stimulates the expression of pro-allergic cytokines (IL-1α, IL-33). Subtilisin induces prototypic allergic lung inflammation. Subtilisin exhibits anticancer activity against breast and colon cancer. Subtilisin shows antifouling activity. Subtilisin can be used as a detergent additive.
  • HY-108910B
    TLCK-treated Chymotrypsin 9004-07-3  
    TLCK-treated Chymotrypsin is a serine protease. Chymotrypsin cleaves protein chains at the carboxyl side of aromatic amino acids. TLCK treated to inactivate residual tryspin activity.
  • HY-E70202
    Recombinant enterokinase 9014-74-8  
    Recombinant enterokinase (rEK) is a serine protease and functions as the physiological activator of trypsinogen. Recombinant enterokinase plays a role of turning trypsinogen to its active form trypsin.