1. Enzyme
  2. Glycobiology Enzymes
  3. Endoproteinase Lys-C

Endoproteinase Lys-C is a protease that cleaves proteins on the C-terminal side of lysine residues and is commonly used for protein sequencing.

For research use only. We do not sell to patients.

Endoproteinase Lys-C Chemical Structure

Endoproteinase Lys-C Chemical Structure

CAS No. : 72561-05-8

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Based on 1 publication(s) in Google Scholar

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Endoproteinase Lys-C is a protease that cleaves proteins on the C-terminal side of lysine residues and is commonly used for protein sequencing[1].

In Vitro

This product can be used for: 1) protein sequence analysis; 2) synthesis of lysine-related derivatives.

This product can be directly used for protein enzyme digestion. The recommended enzyme digestion ratio is 1:50-1:200, and the enzyme digestion should be performed at 37 ℃ overnight.

1) The optimal reaction pH of this product is 9.0-9.5, and the optimal reaction temperature is 30-37 ℃. It can still maintain high enzyme activity in the presence of 4 M urea or 0.1% SDS.
2) Buffer: 20 mM Tris-HCl, pH=8.0.
3) Do not freeze and thaw repeatedly.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.




White to off-white


[Endoproteinase Lys-C]


Room temperature in continental US; may vary elsewhere.


Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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Endoproteinase Lys-C Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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