Advanced MEM Reduced-Serum Medium, no L-Glutamine, no HEPES
MCE Advanced MEM Reduced-Serum Medium (with Phenol Red, Sodium Pyruvate, and Non-Essential Amino Acids; without L-Glutamine or HEPES) is a reduced-serum medium optimized from classic Minimum Essential Medium (MEM) for the routine culture and proliferation of various mammalian cells.
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Storage :
2–8°C, 1 year. Protect from light.
- Manual (1680KB)
- COA
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Description & Advantages
MCE Advanced MEM Reduced-Serum Medium (with Phenol Red, Sodium Pyruvate, and Non-Essential Amino Acids; without L-Glutamine or HEPES) is a reduced-serum medium optimized from classic Minimum Essential Medium (MEM) for the routine culture and proliferation of various mammalian cells. Compared with conventional MEM, it can reduce fetal bovine serum (FBS) use by approximately 50%–90% while maintaining normal cell growth rate and morphology. This helps reduce variation between serum lots, improve culture consistency, and lower culture costs.
| (+) | (-) |
|---|---|
| D-Glucose (1.0 g/L) | L-Glutamine |
| Non-Essential Amino Acids (NEAA) | HEPES |
| Phenol Red (10 mg/L) | |
| Sodium Pyruvate (110.0 mg/L) |
Protocols
MCE Advanced MEM Reduced-Serum Medium (without L-Glutamine or HEPES) contains ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin, and trace elements, including sodium selenite, ammonium metavanadate, copper sulfate, and manganese chloride. These components help reduce the amount of fetal bovine serum required while maintaining normal cell growth rate and morphology. Before use, aseptically add 1%–5% fetal bovine serum (FBS) and supplement with either L-Glutamine (Cat. No.: HY-K1046) or a stabilized L-Alanyl-L-Glutamine supplement (Cat. No.: HY-K1046P) to a final concentration of 4 mM. Mix thoroughly before use.
Application
MCE Advanced MEM Reduced-Serum Medium (without L-Glutamine or HEPES) is suitable for culturing various adherent or semi-adherent mammalian cells. Its use has been reported for MDCK, Vero, PK-15, Hep-G2, HK-2, Caco-2, NCTC Clone 929, HeLa, U-87 MG, HMC3, and other cells. Because serum and nutrient requirements vary by cell type, small-scale optimization is recommended before routine use.
Storage
2–8°C, 1 year.
Protect from light.
Attention
1. This product has been sterilized by filtration. Use aseptic technique to avoid contamination.
2. Not all cells are suitable for reduced-serum culture. Before switching to this medium, perform a small-scale preliminary test to assess cell morphology, viability, and proliferation. Optimize the fetal bovine serum concentration for each cell line, reducing serum use as much as possible while maintaining normal cell growth.
3. When using this product for cell culture, place the cells in a temperature-controlled incubator with 5%–10% CO₂ to maintain an appropriate growth environment.
4. This product is intended for scientific research by trained personnel only. It must not be used for clinical diagnosis or treatment, or in food or pharmaceutical products.
Components
| Components | HY-K3045-500 mL | HY-K3045-3 L |
|---|---|---|
| Advanced MEM Reduced-Serum Medium, no L-Glutamine, no HEPES | 500 mL | 500 mL×6 |
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