AntiFade Mounting Medium (with DAPI)
Based on 22 publication(s) in Google Scholar
Anti-Fade Mounting Medium (with DAPI) is a mounting reagent designed to retard fluorescence photobleaching and preserve signal intensity during long-term imaging. For immediate observation of samples, please select HY-K1048, a non-solidifying anti-fade mounting medium with DAPI. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
4°C, 2 years.
Protect from light.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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HY-K1042
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Phosphatase Inhibitor Cocktail I (100× in DMSO) -
HY-K1001
RIPA Lysis Buffer (Strong) -
HY-18739
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Description & Advantages
MCE AntiFade Mounting Medium (with DAPI) is a sealing reagent used to slow the fluorescence quenching. This reagent is suitable for immobilizing permeabilized cells or tissues, and can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect. This reagent contains DAPI, and can be used for DAPI staining of cell nuclei while sealing the sections.
Protocol
1. After fixation, permeabilization, and immunofluorescence staining of cells or tissues, complete the final wash with PBS or the appropriate wash buffer.
2. Carefully remove excess liquid around the specimen without allowing the sample to dry completely.
3. Apply an appropriate amount of AntiFade Mounting Medium (with DAPI) to fully cover the observation area.
4. Slowly place a coverslip over the sample, avoiding bubbles and allowing the mounting reagent to spread evenly.
5. Observe with a fluorescence or confocal microscope protected from light. DAPI counterstains nuclei during mounting.
Note: This product hardens to form a permanent rigid seal; after hardening, additional edge sealing such as nail polish is generally unnecessary, making it suitable for long-term storage and imaging. Protect the sample and reagent from light throughout the procedure.
Publications
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Journal Impact Factor
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Most Recent
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Mol CancerHsa_circ_0038737 promotes PARPi resistance in castration-resistant prostate cancer via IGF2BP3-mediated DNPH1 mRNA stabilization. [Abstract]2025 Oct 2;24(1):238. PMID: 41039575
AntiFade Mounting Medium (with DAPI) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 Oct 2;24(1):238. [Abstract]
Subcellular localization of hsa_circ_0038737 in 22RV1 and PC-3 cells.
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EMBO Mol Medcirc-EGFR is a predictor of response to Cetuximab and a potential target in colorectal cancer. [Abstract]2025 Dec;17(12):3525-3554. PMID: 41214390
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Int J Mol SciTargeted Therapy of Antibody-Induced Autoimmune Arthritis Using Peptide-Guided Nanoparticles. [Abstract]2024 Nov 8;25(22):12019. PMID: 39596089
AntiFade Mounting Medium (with DAPI) purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Nov 8;25(22):12019. [Abstract]
Hind paws of mice having CAIA were harvested, frozen in OCT, and processed for immunohistochemistry analysis using anti-mouse CD31 antibody conjugated with PE (red), followed by staining with peptide ART-2 conjugated with FITC (green). Also shown is staining by DAPI, a nuclear stain, and the overlay image.
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Scand J Rheumatol2025 May;54(3):198-203. PMID: 39530865
Storage
4°C, 2 years.
Protect from light.
Components
| Components | HY-K1047-5 mL | HY-K1047-25 mL |
|---|---|---|
| AntiFade Mounting Medium (with DAPI) | 5 mL | 5 mL×5 |
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