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Annexin V-FITC/PI Apoptosis Detection Kit

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Based on 87 publication(s) in Google Scholar

MCE Annexin V-FITC/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence(Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).


  • Storage :
    -20°C, 1 year. Protect from light. Avoid repetitive freeze-thaw cycles.
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Annexin V-FITC/PI Apoptosis Detection Kit Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

MCE Annexin V-FITC/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. In normal live cells, Phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane. Upon initiation of apoptosis, PS translocates from the inner to the outer leaflet of the membrane. Annexin V is a 35-36 kDa Ca2+- dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with green fluorescent FITC can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium Iodide (PI) is a cell-membrane impermeable dye to live cells and early apoptosis cells, but stains late apoptosis cells and necrosis cells with red fluorescence. After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).


Protocol

1. Collect 1-5 × 105 cells.

For suspension cells: Centrifuge at 1000 g for 5 minutes and then discard the supernatant. Add 1 mL of pre-cooled PBS to resuspend the cells, centrifuge at 1000 g for 5 minutes and then discard the supernatant.

For adherent cells: Collect the cell culture medium. Wash cells with PBS and add trypsin to dissociate cells. Add the medium and gently suspend the cells to make a single-cell suspension. Centrifuge at 1000 g for 5 minutes and then discard the supernatant. Add 1 mL of pre-cooled PBS to resuspend the cells, centrifuge at 1000 g for 5 minutes and then discard the supernatant.

Note: It is recommended to use trypsin containing no EDTA.

2. Resuspend the cells in 195 μL of Binding Buffer.

3. Add 5 μL of Annexin V-FITC.

4. Add 10 μL of PI Stain.

5. Incubate the cells at room temperature for 10-20 minutes in the dark.

6. Detection by flow cytometer

6.1 Analyze Annexin-FITC binding by flow cytometer (Ex = 488 nm; Em = 525 nm) using FITC signal detector (usually FL1) and PI staining by the phycoerythrin emission signal detector (usually FL2 or FL3).

Note: It is recommended to perform three controls: a: cells with no Annexin-FITC or PI; b: cells with only Annexin-FITC; c: cells with only PI.

6.2 Detection by fluorescence microscope

Fluorescence microscopy: Centrifuge the stained cells at 1,000 g for 5 min, discard the supernatant. Gently resuspend the cell pellet in 50–100 μL of Binding Buffer. Prepare a cell smear and observe and analyze the fluorescence signal using a fluorescence microscope. If using a microplate for observation, gently resuspend the cell pellet in an appropriate volume of Binding Buffer according to the type of microplate used (e.g., 200 μL for a 24-well plate). Transfer the cell suspension to the microplate and observe and analyze the fluorescence signal using a fluorescence microscope.

Storage

-20°C, 1 year.


Protect from light.


Avoid repetitive freeze-thaw cycles.


Components

Components HY-K1073-20 T HY-K1073-50 T HY-K1073-100 T
Annexin V-FITC 100 μL 250 μL 500 μL
Binding Buffer 12 mL 30 mL 60 mL
PI Stain 220 μL 550 μL 1.1 mL

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  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Jul 30.

    Apoptosis fluorescence imaging of MDA-MB-231 cells after different treatments. Scale bar: 50 μm.

  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Jul 30.

    Flow cytometry analysis of apoptosis in MDA-MB-231 cells after different treatments, with Annexin V-FITC fluorescence intensity on the x-axis and PI fluorescence intensity on the y-axis.

  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Dec 26;43(1):330. [Abstract]

    Transfected 143B cells were pretreated with 10 μM fer-1 or Z-VAD-FMK for 1 h and then treated with cisplatin (10 μM) for 24 h; apoptosis levels were assessed by fow cytometry (n=3).

  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Dec 26;43(1):330. [Abstract]

    Transfected 143B cells were pretreated with 10 μM NAC for 1 h and then treated with cisplatin (10 μM) for 24 h. Apoptosis levels were assessed via fow cytometry (n=3).

  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: Biomaterials. 2024 Apr:306:122502. [Abstract]

    Flow cytometric apoptosis analysis of 5/8H/R AC16 cells. The X axis shows Annexin-V-FITC and the Y axis shows PI. A representative cell image is shown for each quadrant.

Assay Types
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
    1/16

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Jul 30.

    Apoptosis fluorescence imaging of MDA-MB-231 cells after different treatments. Scale bar: 50 μm.

  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
    1/16

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Jul 30.

    Flow cytometry analysis of apoptosis in MDA-MB-231 cells after different treatments, with Annexin V-FITC fluorescence intensity on the x-axis and PI fluorescence intensity on the y-axis.

  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
    1/16

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Dec 26;43(1):330. [Abstract]

    Transfected 143B cells were pretreated with 10 μM fer-1 or Z-VAD-FMK for 1 h and then treated with cisplatin (10 μM) for 24 h; apoptosis levels were assessed by fow cytometry (n=3).

  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
    1/16

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Dec 26;43(1):330. [Abstract]

    Transfected 143B cells were pretreated with 10 μM NAC for 1 h and then treated with cisplatin (10 μM) for 24 h. Apoptosis levels were assessed via fow cytometry (n=3).

  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
    1/16

    Annexin V-FITC/PI Apoptosis Detection Kit purchased from MedChemExpress. Usage Cited in: Biomaterials. 2024 Apr:306:122502. [Abstract]

    Flow cytometric apoptosis analysis of 5/8H/R AC16 cells. The X axis shows Annexin-V-FITC and the Y axis shows PI. A representative cell image is shown for each quadrant.

  • Annexin V-FITC/PI Apoptosis Detection Kit Product Image
    1/16
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Experimental Validation Results for Annexin V-FITC/PI Apoptosis Detection Kit
  • Annexin V-FITC/PI Apoptosis Detection Kit Product Image