AntiFade Reagent (with DAPI)
Based on 9 publication(s) in Google Scholar
AntiFade Reagent (with DAPI) is used to slow the fluorescence quenching. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
4℃, 2 years. Protect from light.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Description & Advantages
MCE AntiFade Reagent (with DAPI) is used to slow the fluorescence quenching. The reagent can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect. This product contains DAPI for DAPI staining of cell nucleus.
Protocol
1. After fixation, permeabilization, and immunofluorescence staining of cells or tissues, complete the final wash with PBS or the appropriate wash buffer.
2. Carefully remove excess liquid around the specimen without allowing the sample to dry completely.
3. Apply an appropriate amount of AntiFade Mounting Medium (with DAPI) to fully cover the observation area.
4. Slowly place a coverslip over the sample, avoiding bubbles and allowing the mounting reagent to spread evenly.
5. Observe with a fluorescence or confocal microscope protected from light. DAPI counterstains nuclei during mounting.
Note: This product hardens to form a permanent rigid seal; after hardening, additional edge sealing such as nail polish is generally unnecessary, making it suitable for long-term storage and imaging. Protect the sample and reagent from light throughout the procedure.
Publications
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Journal Impact Factor
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Most Recent
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Cancer CellFusobacterium nucleatum facilitates anti-PD-1 therapy in microsatellite stable colorectal cancer. [Abstract]2024 Oct 14;42(10):1729-1746.e8. PMID: 39303724
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ACS NanoA Cyclized Bivalent Aptamer-Based Protein Degrader Targeting Receptor Tyrosine Kinases Overcomes Resistance to Inhibitors in Lung Cancer. [Abstract]2025 Aug 12;19(31):28171-28185. PMID: 40728393
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J Hazard Mater2024 Dec 5:480:136351. PMID: 39488976
Storage
4℃, 2 years.
Protect from light.
Components
| Components | HY-K1048-5 mL | HY-K1048-25 mL |
|---|---|---|
| AntiFade Reagent (with DAPI) | 5 mL | 5 mL×5 |
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