Phosphatase Inhibitor Cocktail I (100× in DMSO)
Based on 743 publication(s) in Google Scholar
Phosphatase Inhibitor Cocktail I (100× in DMSO) is a blend of 2 phosphatase inhibitors that can effectively inhibit alkaline phosphatases and serine/threonine phosphatases. It is used to maintain the phosphorylation status of proteins.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
-20℃, 2 years.
Description & Advantages
Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases.
MCE Phosphatase Inhibitor Cocktail I (100× in DMSO) is a blend of 2 phosphatase inhibitors that can effectively inhibit alkaline phosphatases and serine/threonine phosphatases. It is used to maintain the phosphorylation status of proteins. This product can be applied in Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, Kinase assay and etc.
Price Comparison
Protocol
Thaw at room temperature, add the inhibitor mixture to the lysate at 1:100 (v/v), and mix thoroughly.
Publications
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Journal Impact Factor
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Most Recent
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Signal Transduct Target TherTargeting cancer cell plasticity by HDAC inhibition to reverse EBV-induced dedifferentiation in nasopharyngeal carcinoma. [Abstract]2021 Sep 4;6(1):333. PMID: 34482361
Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Sep 4;6(1):333. [Abstract]
Phosphatase Inhibitor Cocktail I (30 min).CNE1/HNE2/CNE2-TetOn-LMP1 and CNE1/HNE2/CNE2-TetOn-Vector cells were treated by 100 ng/ml Dox, or knockdown of LMP1 in C666-1 cells for 48 hours, and then the expression of CEBPA was measured by immunoblotting.
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Signal Transduct Target Ther2021 Aug 30;6(1):322. PMID: 34462423
Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Aug 30;6(1):322. [Abstract]
Western blot analysis showed overexpression of VAV2 and γ-H2AX in ESCC cells treated with radiation (10 Gy), which is time- and dose-dependent.
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Cancer Cell2024 Jun 10;42(6):968-984.e9. PMID: 38788719
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ImmunityMetabolic regulator LKB1 controls adipose tissue ILC2 PD-1 expression and mitochondrial homeostasis to prevent insulin resistance. [Abstract]2024 Jun 11;57(6):1289-1305.e9. PMID: 38772366
Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Jun 11;57(6):1289-1305.e9. [Abstract]
Western blot analysis of phosphorylated Akt (P-Akt, Ser473) and total Akt in tissues.
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Cancer Commun (Lond)Combining radiation and the ATR inhibitor berzosertib activates STING signaling and enhances immunotherapy via inhibiting SHP1 function in colorectal cancer. [Abstract]2023 Apr;43(4):435-454. PMID: 36855844
Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Apr;43(4):435-454. [Abstract]
Immunoblot analysis of γH2AX and pCHK1 in multiple CRC cell lines treated with different regimens.
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Nat NeurosciEmergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus. [Abstract]2023 May;26(5):751-764. PMID: 36973513
Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MedChemExpress. Usage Cited in: Nat Neurosci. 2023 May;26(5):751-764. [Abstract]
Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group.
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Storage
-20℃, 2 years.
Components
| Cat. No. | Product Name | Package |
|---|---|---|
| HY-K0021-1 mL | Phosphatase Inhibitor Cocktail Ⅰ (100× in DMSO) | 1 mL |
| HY-K0021-10 mL | Phosphatase Inhibitor Cocktail Ⅰ (100× in DMSO) | 1 mL × 10 |
| HY-K0021-50 mL | Phosphatase Inhibitor Cocktail Ⅰ (100× in DMSO) | 1 mL × 50 |
| HY-K0021-100 mL | Phosphatase Inhibitor Cocktail Ⅰ (100× in DMSO) | 1 mL × 100 |