1. MCE Kits
  2. Protein Biology
  3. Protein Sample Preparation
  4. Phosphatase Inhibitor Cocktail
  5. Phosphatase Inhibitor Cocktail I

Phosphatase Inhibitor Cocktail I  (100× in DMSO)

Cat. No.: HY-K0021
Manual COA SDS

Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.

Phosphatase Inhibitor Cocktail I
Size (Pre-dissolved in DMSO) Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 45 In-stock
10 mL USD 315 In-stock
50 mL USD 1330 In-stock
100 mL USD 1990 In-stock

* Please select Quantity before adding items.

  • Description

  • Price Comparison

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 µM), and Microcystin-LR , Microcystis aeruginosa (500 nM).

Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.

Publications
Price Comparison
Phosphatase Inhibitor Cocktail I Price Comparison
Storage
The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.
Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components
Cat. No. Product Name Package
HY-K0021-1 mL Phosphatase Inhibitor Cocktail I (100× in DMSO) 1 mL
HY-K0021-10 mL Phosphatase Inhibitor Cocktail I (100× in DMSO) 1 mL × 10
HY-K0021-50 mL Phosphatase Inhibitor Cocktail I (100× in DMSO) 1 mL × 50
HY-K0021-100 mL Phosphatase Inhibitor Cocktail I (100× in DMSO) 1 mL × 100
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Phosphatase Inhibitor Cocktail I
Cat. No.:
HY-K0021
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