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  5. Phosphatase Inhibitor Cocktail
  6. Phosphatase Inhibitor Cocktail I (100× in DMSO)

Phosphatase Inhibitor Cocktail I (100× in DMSO) 

Cat. No.: HY-K0021
Manual COA SDS Technical Support
Cocktails

Phosphatase Inhibitor Cocktail I (100× in DMSO) is a blend of 2 phosphatase inhibitors that can effectively inhibit alkaline phosphatases and serine/threonine phosphatases. It is used to maintain the phosphorylation status of proteins.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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675 Publications Citing Use of MCE Phosphatase Inhibitor Cocktail I (100× in DMSO)

WB

    Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MCE. Usage Cited in: Immunity. 2024 Jun 11;57(6):1289-1305.e9.  [Abstract]

    Western blot analysis of phosphorylated Akt (P-Akt, Ser473) and total Akt in tissues.

    Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 Apr;43(4):435-454.  [Abstract]

    Immunoblot analysis of γH2AX and pCHK1 in multiple CRC cell lines treated with different regimens.

    Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MCE. Usage Cited in: Nat Neurosci. 2023 May;26(5):751-764.  [Abstract]

    Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group.

    Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2021 Sep 4;6(1):333.  [Abstract]

    Phosphatase Inhibitor Cocktail I (30 min).CNE1/HNE2/CNE2-TetOn-LMP1 and CNE1/HNE2/CNE2-TetOn-Vector cells were treated by 100 ng/ml Dox, or knockdown of LMP1 in C666-1 cells for 48 hours, and then the expression of CEBPA was measured by immunoblotting.

    Phosphatase Inhibitor Cocktail I (100× in DMSO) purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2021 Aug 30;6(1):322.  [Abstract]

    Western blot analysis showed overexpression of VAV2 and γ-H2AX in ESCC cells treated with radiation (10 Gy), which is time- and dose-dependent.

    • Description

    • Price Comparison

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases.

    MCE Phosphatase Inhibitor Cocktail I (100× in DMSO) is a blend of 2 phosphatase inhibitors that can effectively inhibit alkaline phosphatases and serine/threonine phosphatases. It is used to maintain the phosphorylation status of proteins. This product can be applied in Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, Kinase assay and etc.
    Price Comparison
    Phosphatase Inhibitor Cocktail I (100× in DMSO) Price Comparison
    Storage

    -20℃, 2 years
    Protocol

    Thaw at room temperature, add the inhibitor mixture to the lysate at 1:100 (v/v), and mix thoroughly.
    Components
    Cat. No. Product Name Package
    HY-K0021-1 mL Phosphatase Inhibitor Cocktail Ⅰ (100× in DMSO) 1 mL
    HY-K0021-10 mL Phosphatase Inhibitor Cocktail Ⅰ (100× in DMSO) 1 mL × 10
    HY-K0021-50 mL Phosphatase Inhibitor Cocktail Ⅰ (100× in DMSO) 1 mL × 50
    HY-K0021-100 mL Phosphatase Inhibitor Cocktail Ⅰ (100× in DMSO) 1 mL × 100
    Documentation

    Manual (976 KB) SDS (311 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Protein Degradation InhibitorProtein inhibitor

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