1. MCE Kits
  2. Phosphatase Inhibitor Cocktail I

Phosphatase Inhibitor Cocktail I  (100× in DMSO)

Cat. No.: HY-K0021
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Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.

Phosphatase Inhibitor Cocktail I
Size (Pre-dissolved in DMSO) Price Stock Quantity
Free Sample   Apply now  
1  mL USD 45 In-stock
Estimated Time of Arrival: December 31
1  mL × 10 USD 315 In-stock
Estimated Time of Arrival: December 31
1  mL × 50 USD 1330 In-stock
Estimated Time of Arrival: December 31
1  mL × 100 USD 1990 In-stock
Estimated Time of Arrival: December 31

* Please select Quantity before adding items.

Top Publications Citing Use of Products

Customer Validation

  • Protein: Bcl-2

    Cell Line: thyroid cancer cell line 8505C

    MCE Protease Inhibitor Cocktail provides Bcl-2 protection in thyroid cancer cell line 8505C, and is most effective after 6-12 hours incubation at room temperature.

  • Protein: ERK1/2

    Cell Line: K1 cell line

    MCE Protease Inhibitor Cocktail (mini-Tablet) protects ERK1/2 in K1 cell line, and is most effective after 6-12 hours incubation at room temperature.

MCE Protease Inhibitor Cocktail provides Bcl-2 protection in thyroid cancer cell line 8505C, and is most effective after 6-12 hours incubation at room temperature.

 

MCE Protease Inhibitor Cocktail (mini-Tablet) protects ERK1/2 in K1 cell line, and is most effective after 6-12 hours incubation at room temperature.

 
  • Description

  • Price Comparison

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 µM), and Microcystin-LR , Microcystis aeruginosa (500 nM).

Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.

Publications
Price Comparison
Phosphatase Inhibitor Cocktail I Price Comparison
Storage
The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.
Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components
Ingredient M.W. 100x Conc. Target
(-)-p-Bromotetramisole oxalate 995.2 2.5 mM Alkaline phosphatases
Cantharidin 373.2 500 μM Ser/Thr phosphatases
Microcystin LR, Microcystis aeruginosa 196.2 500 nM PP1 and PP2A
Documentation

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Product Name:
Phosphatase Inhibitor Cocktail I
Cat. No.:
HY-K0021
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