Basement Membrane Matrix HC (Phenol Red)
Based on 1 publication(s) in Google Scholar
MCE Basement Membrane Matrix HC (High Concentration, Phenol Red) is primarily composed of natural basement membrane matrix extracted from mouse tumorsand contains abundant extracellular matrix components and a variety of naturally occurring growth factors. This product is mainly used for angiogenesis experiments, construction of animal models and 3D tumor models.
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Storage :
-20°C, 2 years.
Avoid repeated freezing and thawing.
Shipping with dry ice
Description & Advantages
Basement Membrane Matrix is a natural basement membrane extract derived from mouse tumor tissue rich in extracellular matrix (ECM) proteins. It mimics the in vivo cellular microenvironment and provides structural support for cell growth. It contains abundant extracellular matrix components, including laminin, type IV collagen (Collagen IV), entactin, heparan sulfate proteoglycans (HSPGs), and others. It also contains a variety of naturally occurring growth factors, including insulin-like growth factor-1 (IGF-1), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and others.
MCE High Concentration Basement Membrane Matrix (with Phenol Red) has a higher protein concentration and greater gel strength than standard Basement Membrane Matrix, providing a more stable three-dimensional support structure. It is suitable for in vivo model establishment and provides a microenvironment that more closely resembles physiological conditions for cell growth, differentiation, and tissue formation, thereby enabling more accurate simulation of in vivo conditions and improving the physiological relevance and reliability of experimental results.
Protocol
Recommended Application Methods
MCE High Concentration Basement Membrane Matrix (Phenol Red) can be used in the following two application methods.
| Application Method | Primary Function | Common Applications |
|---|---|---|
| Thick Gel | Forms a 1-2 mm three-dimensional gel structure | Angiogenesis assays and aortic ring assays |
| Gel Embedding | Cells are completely embedded within the matrix, providing an in vivo-like three-dimensional microenvironment to promote cell survival, growth, and invasion | Subcutaneous xenograft models |
Preparation Before Experiment
• Slight variations in protein concentration may occur between different batches. Please refer to the corresponding Certificate of Analysis (COA) for specific information.
• To achieve optimal experimental reproducibility, it is recommended to calculate the required volume based on the actual protein concentration and dilute the matrix using pre-chilled serum-free medium.
• Maintain low temperature conditions (ice bath or 4°C) throughout the entire procedure to prevent premature gelation of the basement membrane matrix.
Publications
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Journal Impact Factor
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Most Recent
Storage
-20°C, 2 years.
Avoid repeated freezing and thawing.
Shipping with dry ice
Components
| Components | HY-K6005-1 mL | HY-K6005-5 mL | HY-K6005-10 mL |
|---|---|---|---|
| Basement Membrane Matrix HC (Phenol Red) | 1 mL | 5 mL | 10 mL |