Basement Membrane Matrix HC (Phenol Red) 

•   MCE Basement Membrane Matrix HC (Phenol Red) is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc. The percentage of each of these growth factors is higher than that of standard basement membrane matrix.

•   MCE Basement Membrane Matrix HC (Phenol Red) is mainly used for angiogenesis experiments, animal models and 3D tumor model construction.

-20°C, 2 years.

Avoid repeated freezing and thawing.

a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

b. Dilute MCE Basement Membrane Matrix HC with cell suspension at 4°C in a 1:1 ratio to a suitable final concentration.

c. Inject diluted cell suspension at a depth of 1 cm under the skin on nude mice. The cell suspension should be injected in 30 minutes and be placed on ice to slow down apoptosis and prevent from gelling.

d. Formed tumors can be seen in about 1 week.