CHO Magnetic Beads (200 nm, 10 mg/mL)
Based on 1 Customer Validation
MCE CHO Magnetic Beads (200 nm, 10 mg/mL) contain CHO functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages，can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.
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Storage :
4℃, 2 years. Do not freeze.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE CHO Magnetic Beads (200 nm, 10 mg/mL) contain CHO functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages，can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules. Compared with the traditional carboxyl and amino magnetic beads, CHO magnetic beads do not need to be activated by glutaraldehyde. Simply dissolve the primary amine-containing biological ligands in PBS and incubate at room temperature to covalently couple the bioconjugate to the beads, which is easy to operate, mild coupling conditions, fast and efficient. CHO have the ability to form covalent bonds with amino groups. Therefore, the beads coupling process must be carried out in a buffered solvent without any amine, and automated instruments are especially useful for large screening of multiple samples.
Protocol
Recommended Buffers (Not Provided)
| Buffer | Composition |
|---|---|
| Wash Buffer | PBS, 0.05% Tween-20, pH 7.4 |
| Blocking Buffer | 0.5 M ethanolamine, pH 8.3; or 0.1 M Tris-HCl, 150 mM NaCl, pH 8.5 |
| Storage Buffer | PBS containing 0.02% (w/v) NaN3 (optional) |
Operating Instructions
Coupling of Magnetic Beads to Biomolecules (For Reference Only)
The following procedure is described using 500 μL of magnetic beads, 50-200 μg of biomolecule, and a 1.5 mL EP tube as an example. The quantities may be adjusted proportionally according to experimental requirements.
1. Magnetic Bead Pretreatment
Thoroughly mix the magnetic beads. Transfer 500 μL of magnetic beads into a 1.5 mL EP tube, add 1 mL of wash buffer, and mix thoroughly. Place the tube on a magnetic stand for magnetic separation and discard the supernatant. Repeat the washing procedure twice.
2. Magnetic Bead Coupling
Add 50-200 μg of biological ligand to the magnetic beads. The amount and concentration may be optimized according to the specific experiment, while maintaining the pH of the reaction system at approximately 8.0. Mix gently and incubate on a rotator protected from light for 1-4 h at room temperature or overnight at 4°C.
3. Blocking
Perform magnetic separation and remove the supernatant. Add 500-1000 μL of blocking buffer to resuspend the magnetic beads. Incubate the mixture on a rotator protected from light for 2-4 h at room temperature or overnight at 4°C.
4. Storage
Perform magnetic separation and remove the supernatant. Resuspend the magnetic beads in an appropriate volume of storage buffer. The final volume of storage buffer may be adjusted according to the experimental requirements to obtain the desired concentration of ligand-coupled magnetic beads. Store at 4°C.
Storage
4℃, 2 years.
Do not freeze.
Components
| Components | HY-K0226-1 mL | HY-K0226-5 mL | HY-K0226-10 mL |
|---|---|---|---|
| CHO Magnetic Beads (200 nm, 10 mg/mL) | 1 mL | 5 mL | 10 mL |
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