HAT Supplement (50×)
Based on 1 Customer Validation
MCE HAT Supplement (50×) can be added to cell culture medium as a selection agent for post-fusion selection of HGPRT-myeloma hybridoma cells used for antibody production. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
-20℃, 1 year
Keep away from light
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
HAT Supplement can be used to specific selection of HGPRT-myeloma hybridoma cells. The aminopterin inhibits the de novo nucleoside biosynthesis pathway. Hypoxanthine and thymidine supply purines and pyrimidines for DNA synthesis by hybridoma cells via the salvage pathway that utilizes HGPRT contributed by the fused spleen cell. Unfused HGPRT-deficient myeloma cells cannot replicate DNA to proliferate, and unfused spleen cells are mortal, allowing for specific selection of HGPRT-myeloma hybridoma cells.
MCE HAT Supplement (50×) is a liquid mixture of sodium aminopterin (20 μM), hypoxanthine (5 mM), and thymidine (0.8 mM). HAT Supplement can be added to cell culture medium as a selection agent for post-fusion selection of HGPRT-myeloma hybridoma cells used for antibody production.
Protocol
1. Thaw MCE HAT Supplement (50×) at room temperature in advance.
2. Take the appropriate amount of HAT Supplement (50×) in a sterile environment and add it to the cell culture medium at a 50-fold dilution according to the experimental requirements. For example: add 1 mL of supplement to 49 mL of medium.
Storage
-20℃, 1 year
Keep away from light
Attention
1. This product is a 50× stock solution. Please dilute it as needed before use.
2. This product appears as a colorless and transparent liquid, with an endotoxin content of ≤ 0.25 EU/mL.
3. Precipitation may occur after thawing, dissolve it completely in a 37°C water bath.
4. It is recommended to appropriately aliquot the solution after thawing to avoid repeated freeze-thaw cycles.
5. Pay attention to aseptic operation to avoid contamination.
6. This product is for R&D use only, not for drug, household, or other uses.
7. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K3016-10 mL | HY-K3016-100 mL |
|---|---|---|
| HAT Supplement (50×) | 10 mL | 10 mL × 10 |
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