2. MCE-Kits
  3. Cell Biology
  4. 3D Cell Culture
  5. Basement Membrane Matrix
  6. Basement Membrane Matrix

Basement Membrane Matrix  

Art. -Nr.: HY-K6002
Manual COA SDS Technical Support
Organoid Culture

MCE Basement Membrane Matrix is primarily composed of natural basement membrane matrix extracted from mouse tumors.This product is mainly used for studies of tumor invasion, angiogenesis and organoids culture while avoiding color interference in subsequent experiments.

Größe Preis Verfügbarkeit Menge
Kostenlose Probe (1 mL)   Jetzt bewerben  
1 mL Auf Lager
5 mL Auf Lager
10 mL Auf Lager
or Massenanfrage

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

    Basement Membrane Matrix purchased from MCE. Usage Cited in: J Hazard Mater. 2025 May 26:494:138736.  [Abstract]

    Representative images: thHTR8/SVneo cells were treated with different concentrations of NaAsO2 (0.02, 0.2, 2 μM) for 12 days.

    Basement Membrane Matrix purchased from MCE. Usage Cited in: FASEB J. 2024 Jul 15;38(13):e23806.  [Abstract]

    Tube formation capacity was assessed by tube formation assay after ox-LDL treatment.

      MedChemExpress Validation
      Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
      Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
      Matrix gel: 8 μL
      Complete culture medium: 50 μL
      Under identical tube formation assay conditions, MCE matrix gel (Basement Membrane Matrix, HY-K6002) demonstrated superior tube-forming performance compared with competitor brands at both undiluted and 2:1 dilution conditions. The tubular structures formed with MCE matrix gel were more complete and continuous, with clearer and more stable network architecture.

      MedChemExpress Validation
      Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
      Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
      Matrix gel: 8 μL
      Complete culture medium: 50 μL
      Under identical tube formation assay conditions, the tube formation effects of different batches of MCE Matrigel (Basement Membrane Matrix, HY-K6002) were compared under both undiluted and 2:1 diluted conditions.Different batches of MCE Matrigel exhibited consistent tube formation ability. The formed tubular structures were intact and continuous, with clear and stable network structures.The overall tube formation effect was stable and reproducible, with minor differences between batches, indicating good batch-to-batch stability.

      MedChemExpress Validation
      Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
      Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
      Matrix gel: 8 μL
      Complete culture medium: 50 μL
      Under identical tube formation assay conditions, the tube formation effects of MCE Matrigel (Basement Membrane Matrix, HY-K6002) and competitor brand Matrigel were compared under both undiluted and 2:1 diluted conditions. MCE Matrigel exhibited superior tube formation ability, with more intact and continuous tubular structures and clearer, more stable network structures.

      • Beschreibung

      • Speicherung

      • Protokoll

      • Komponenten

      • Dokumentation

      Description
      & Advantages

      MCE Basement Membrane Matrix is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc. The concentration of each component in MCE basement membrane matrix conforms to the standard type range.

      MCE Basement Membrane Matrix is suitable for studies of tumor invasion, angiogenesis and organoids culture while avoiding color interference in subsequent experiments.
      Speicherung

      -20°C, 2 years.

      Avoid repeated freezing and thawing.

      Shipping with dry ice
      Protokoll

      a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

      b. Use pre-cooled serum-free medium to dilute according to experimental requirements. Refer to the Experimental Conditions table for final concentrations.

      c. Place the culture plate on ice and inject the substrate gel dilution slowly against the wall to avoid air bubbles. Refer to the Experimental Conditions table for the volume injected.

      d. Transfer the culture plate to 37℃ incubator gently. After gelling, aspirate off excess supernatant. Refer to the Experimental Conditions table for gel time.

       

      Note: When thawing the product for the first time, immerse the vial in crushed ice and place it in a 4°C to melt. After melting, mix thoroughly before aliquoting, and then store at -20°C.
      Komponenten
      Components HY-K6002-1 mL HY-K6002-5 mL HY-K6002-10 mL
      Basement Membrane Matrix 1 mL 5 mL 10 mL
      Dokumentation

      Manual (4348 KB) SDS (105 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

      Keywords:

      Matrix-GelMatrigelExtracellular matrixscaffolds

      Ihre kürzlich angesehenen Produkte:

      Massenanfrage

      Your information is safe with us. * Required Fields.

      Produktname

      		  

      Requested Quantity *

      Name des Antragstellers *

      		 

      Anrede

      E-Mail-Addresse *

      Telefonnummer *

      Department

      		 

      Name der Organisation *

      City

      Bundesland

      Country or Region *

      		      

      Bemerkungen

      Massenanfrage

      Inquiry Information

      Produktname:
      Basement Membrane Matrix
      Art. -Nr.:
      HY-K6002
      Menge:
      MCE Japan Authorized Agent:

      Request for COA Report

      Please fill out this form to request the COA report. We will send it to your email within 3 working days. Your information is safe with us. * Required Fields.

      .

      Product Name

      		  

      Lot # *

      Applicant Name *

      		 

      Email Address *

      Phone Number

      		 

      Department *

      Organization Name *

      		 

      Remarks

      Request for COA Report

      We have received your request and will respond to you as soon as possible.