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  6. SYBR Green qPCR Master Mix (Universal)

SYBR Green qPCR Master Mix (Universal) 

Cat. No.: HY-K0501A
Manual COA SDS Technical Support

MCE SYBR Green qPCR Master Mix (Universal) is compatible with all qPCR instruments. 2× ready-to-use formulations which consist of antibody-mediated hot start polymerase, MgCl2, dNTPs and a proprietary reaction buffer, produce optimal results in qPCR experiments. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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71 Publications Citing Use of MCE SYBR Green qPCR Master Mix (Universal)

RT-PCR

    SYBR Green qPCR Master Mix (Universal) purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373.  [Abstract]

    Chromatin immunoprecipitation (ChIP) assays were performed with an anti-E2F4 antibody or an IgG control. The samples were subjected to qPCR with the indicated primers that recognize the MTDH promoter region.

    SYBR Green qPCR Master Mix (Universal) purchased from MCE. Usage Cited in: Nat Commun. 2025 Apr 1;16(1):3131.  [Abstract]

    qRT-PCR analysis of typical ferroptosis-related markers in the IVDs post-discectomy (n = 6 independent rats).

    SYBR Green qPCR Master Mix (Universal) purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2024 Dec 24:e2405483.  [Abstract]

    Quantified GFAP mRNA levels and GFAP‐positive cells in the SDH of rats at day 14 after nerve injury.

    SYBR Green qPCR Master Mix (Universal) purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2025 Feb;45(2):110-137.  [Abstract]

    mRNA levels of NNMT and AOX1 in tumor and paired normal tissues of LUAD and LUSC tested by RT-qPCR.

    SYBR Green qPCR Master Mix (Universal) purchased from MCE. Usage Cited in: Nat Commun. 2024 Oct 28;15(1):9304.  [Abstract]

    Verification of the DNA concentration and purification by qPCR assay. PC positive control, extracted the genomic DNA of C. albicans using a fungal genomic DNA extraction kit (Solarbio, China). NC: represents reactions without substrate.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    SYBR Green qPCR Master Mix (Universal) utilizes a special performance-enhanced antibody-mediated hot start Taq DNA polymerase and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR (qPCR). MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water. Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer, produce optimal results in qPCR experiments. What's more, with a unique reference dye, this product is compatible with all qPCR instruments.

     

    1. Performance-enhanced antibody-mediated Taq DNA polymerase.

    2. Unique reference dye is compatible with all qPCR instruments.

    3. Optimized buffer enhances specificity and reduces primer-dimer formation.

    4. High sensitivity, specificity and reliability.

    Storage

    -20°C, 2 years

    Protecting from light, and avoid repetitive freeze-thaw cycles
    Components
    Components HY-K0501A-100 rxns HY-K0501A-500 rxns
    SYBR Green qPCR Master Mix (Universal) 1 mL 1 mL × 5
    Documentation

    Manual (115 KB) SDS (254 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SYBR dye

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