SYBR Green qPCR Master Mix (Universal)
Based on 78 publication(s) in Google Scholar
MCE SYBR Green qPCR Master Mix (Universal) is compatible with all qPCR instruments. 2× ready-to-use formulations which consist of antibody-mediated hot start polymerase, MgCl2, dNTPs and a proprietary reaction buffer, produce optimal results in qPCR experiments. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
-20°C, 2 years. Protecting from light, and avoid repetitive freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Description & Advantages
SYBR Green qPCR Master Mix (Universal) utilizes a special performance-enhanced antibody-mediated hot start Taq DNA polymerase and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR (qPCR). MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water. Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer, produce optimal results in qPCR experiments. What's more, with a unique reference dye, this product is compatible with all qPCR instruments.
1. Performance-enhanced antibody-mediated Taq DNA polymerase.
2. Unique reference dye is compatible with all qPCR instruments.
3. Optimized buffer enhances specificity and reduces primer-dimer formation.
4. High sensitivity, specificity and reliability.
Protocol
General Protocol
1. Preparation of the reaction mixture: prepare the reaction mixture on ice. Three technical replicates are recommended for each sample.
| Component | 50 μL reaction | 20 μL reaction | Final concentration |
|---|---|---|---|
| SYBR Green qPCR Master Mix (Universal) | 25 μL | 10 μL | 1× |
| Forward Primer (10 μM) | 1 μL | 0.4 μL | 0.2 μM |
| Reverse Primer (10 μM) | 1 μL | 0.4 μL | 0.2 μM |
| DNA/cDNA | 4 μL | 2 μL | / |
| ddH2O | 19 μL | 7.2 μL | / |
| Total volume | 50 μL | 20 μL | / |
Note: a. A final primer concentration of 200 nM is commonly used and may be optimized within 0.1-1.0 μM according to amplification efficiency and specificity;
b. Recommended template amount: 10-100 ng genomic DNA or 1-10 ng cDNA. Adjust the template amount according to target abundance to keep Ct values preferably within 15-35;
c. It is recommended to prepare a Master Mix without template first, dispense it, and then add template to reduce pipetting error.
d. The Universal formulation does not require addition of a separate ROX Reference Dye.
2. qPCR cycling program:
| Step | Temperature | Time | Cycles |
|---|---|---|---|
| Initial denaturation | 95°C | 30 sec | 1 |
| Denaturation | 95°C | 10 sec | 40 |
| Annealing/Extension | 60°C | 30 sec | 40 |
| Melting curve | Use the instrument default program | - | - |
3. Perform melting-curve analysis after amplification to confirm product specificity. Ramp rates and fluorescence acquisition settings may be adjusted according to the real-time PCR instrument used.
Publications
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Journal Impact Factor
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Most Recent
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Signal Transduct Target TherFamily with sequence similarity 114 member A1 orchestrates immune evasion in triple-negative breast cancer. [Abstract]2025 Nov 18;10(1):373. PMID: 41249116
SYBR Green qPCR Master Mix (Universal) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]
Chromatin immunoprecipitation (ChIP) assays were performed with an anti-E2F4 antibody or an IgG control. The samples were subjected to qPCR with the indicated primers that recognize the MTDH promoter region.
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Cancer Commun (Lond)Nicotinamide N-methyltransferase negatively regulates metastasis-promoting property of cancer-associated fibroblasts in lung adenocarcinoma. [Abstract]2025 Feb;45(2):110-137. PMID: 39623600
SYBR Green qPCR Master Mix (Universal) purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Feb;45(2):110-137. [Abstract]
mRNA levels of NNMT and AOX1 in tumor and paired normal tissues of LUAD and LUSC tested by RT-qPCR.
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Nat CommunInjectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes. [Abstract]2025 Apr 1;16(1):3131. PMID: 40169595
SYBR Green qPCR Master Mix (Universal) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 1;16(1):3131. [Abstract]
qRT-PCR analysis of typical ferroptosis-related markers in the IVDs post-discectomy (n = 6 independent rats).
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Nat CommunAffinity molecular assay for detecting Candida albicans using chitin affinity and RPA-CRISPR/Cas12a. [Abstract]2024 Oct 28;15(1):9304. PMID: 39468064
SYBR Green qPCR Master Mix (Universal) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Oct 28;15(1):9304. [Abstract]
Verification of the DNA concentration and purification by qPCR assay. PC positive control, extracted the genomic DNA of C. albicans using a fungal genomic DNA extraction kit (Solarbio, China). NC: represents reactions without substrate.
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Adv Sci (Weinh)Pathophysiology-Directed Engineering of a Combination Nanoanalgesic for Neuropathic Pain. [Abstract]2025 Feb;12(8):e2405483. PMID: 39716944
SYBR Green qPCR Master Mix (Universal) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(8):e2405483. [Abstract]
Quantified GFAP mRNA levels and GFAP‐positive cells in the SDH of rats at day 14 after nerve injury.
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Proc Natl Acad Sci U S ATranscription factor EGR2 alleviates autoimmune uveitis via activation of GDF15 to modulate the retinal microglial phenotype. [Abstract]2024 Sep 24;121(39):e2316161121. PMID: 39298490
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Int J Biol MacromolGLUT3 coordinates mitochondrial respiration and metabolic reprogramming in ERα-positive breast cancer. [Abstract]2025 Dec 27;339(Pt 1):149934. PMID: 41461228
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Int Immunopharmacol2026 Jan 1;168(Pt 1):115803. PMID: 41218583
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Int J Mol Sci2023 Apr 12;24(8):7136. PMID: 37108300
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Am J Physiol Cell PhysiolNF-кB promotes aggresome formation via upregulating HDAC6 and in turn maintaining Vimentin cage. [Abstract]2024 Nov 1;327(5):C1289-C1299. PMID: 39374080
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Sci RepUTRN as a potential biomarker in breast cancer: a comprehensive bioinformatics and in vitro study. [Abstract]2024 Apr 2;14(1):7702. PMID: 38565593
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Poult SciDynamic changes in genes and metabolites during embryonic development of Jiangshan black-bone chickens. [Abstract]2026 Mar 18;105(6):106783. PMID: 41887166
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Microb PathogTranscriptome analysis of sRNA responses to four different antibiotics in Pseudomonas aeruginosa PAO1. [Abstract]2022 Dec;173(Pt A):105865. PMID: 36332790
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Mol Biol Rep2024 Aug 8;51(1):895. PMID: 39115693
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Hum CellUSF1-induced RPS6KB2 activation influences aggressive phenotype in B-cell non-Hodgkin lymphoma. [Abstract]2025 Aug 31;38(5):154. PMID: 40886264
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Oxid Med Cell Longev2022 Aug 22;2022:1486157. PMID: 36046692
Storage
-20°C, 2 years.
Protecting from light, and avoid repetitive freeze-thaw cycles.
Components
| Components | HY-K0501A-100 rxns | HY-K0501A-500 rxns |
|---|---|---|
| SYBR Green qPCR Master Mix (Universal) | 1 mL | 1 mL × 5 |
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