Lipid Peroxidation (MDA) Assay Kit
Based on 9 publication(s) in Google Scholar
MCE Lipid Peroxidation (MDA) Assay Kit is suitable for measuring MDA levels in a variety of samples including plasma, serum, urine, tissues or cell lysates.
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Storage :
-20℃, 1 year
Keep away from light and avoid repeated freezing and thawing
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Description & Advantages
Lipid peroxidation generally refers to the oxidative degradation of cellular lipids by reactive oxygen species. Peroxidation of unsaturated lipids affects cell membrane properties, signal transduction pathways, apoptosis, and the deterioration of foods and other biological compounds. Lipid peroxidation degradation forms reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). In this process free radicals take electrons from the lipids (generally in cell membranes), resulting in cell damage. Therefore MDA and 4-HNE are often used as markers of lipid peroxidation, and to assay for oxidative damage / oxidative stress.
MCE Lipid Peroxidation (MDA) Assay Kit is suitable for measuring MDA levels in a variety of samples including plasma, serum, urine, tissues or cell lysates. The detection principle is that the MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct (The reaction principle is illustrated in the following figure). The MDA-TBA adduct can be quantified colorimetrically (OD = 535 nm) fluorescent (Ex/Em = 532/553 nm) detection.
Protocol
Reagent Preparation
1. MDA detection working solution: mix MDA Detection Solution and Antioxidant at a volume ratio of 100:1. Prepare immediately before use. Approximately 202 μL working solution is recommended per reaction.
2. MDA standards: dilute the 1 mM MDA Standard Solution with deionized water or the same buffer used for samples to 0, 1, 2, 4, 6, 8, 10, and 20 μM.
Sample Preparation
1. Plasma, serum, or urine: clear samples may be assayed directly. If precipitated or turbid, centrifuge at 10,000 × g for 10 min at 4°C and collect the supernatant.
2. Cells: collect approximately 1 × 106 cells, wash with PBS, add 100-200 μL suitable lysis buffer, lyse thoroughly on ice, centrifuge at 10,000 × g for 10 min at 4°C, and collect the supernatant.
3. Tissue: weigh 10-30 mg tissue, add 100 μL PBS or suitable homogenization buffer per 10 mg tissue, homogenize on ice, centrifuge at 10,000 × g for 10 min at 4°C, and collect the supernatant.
Assay Procedure
1. Add 100 μL MDA standard or sample supernatant to a 1.5 mL centrifuge tube.
2. Add 200 μL MDA detection working solution to each tube and mix thoroughly.
3. Heat at 95°C for 40 min in a water bath or heating block.
4. Immediately cool in an ice bath for 10 min.
5. Centrifuge at 1,000 × g for 10 min and transfer 200 μL supernatant to a clear 96-well plate.
6. Measure absorbance at 532 nm using a microplate reader.
Calculation
Subtract the blank absorbance, plot MDA concentration against absorbance to generate a standard curve, and determine MDA concentrations in samples. Multiply by the dilution factor and normalize to protein content, cell number, or tissue weight as required.
Publications
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Journal Impact Factor
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Most Recent
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Clin Exp Hypertens2026 Dec 31;48(1):2607404. PMID: 41447318
Lipid Peroxidation (MDA) Assay Kit purchased from MedChemExpress. Usage Cited in: Clin Exp Hypertens. 2026 Dec 31;48(1):2607404. [Abstract]
Quantification of intracellular MDA levels in H9c2 cells. Malondialdehyde (MDA) content was quantified according to the manufacturer's instructions of the Lipid Peroxidation (MDA) Assay Kit.
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Mol Med RepIdentification of the clinical value and biological effects of TTN mutation in liver cancer. [Abstract]2025 Jun;31(6):165. PMID: 40242970
Lipid Peroxidation (MDA) Assay Kit purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 Jun;31(6):165. [Abstract]
MDA content in Huh7 and Hep3B cells after TTN overexpression and 5‑FU treatment.Malondialdehyde (MDA) content was quantified according to the manufacturer's instructions of the Lipid Peroxidation (MDA) Assay Kit.
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Pestic Biochem PhysiolChaetoglobosin D isolated from the endophytic fungus MG2 exhibited a good control to apple tree Valsa canker. [Abstract]2025 Sep:213:106563. PMID: 40744597
Lipid Peroxidation (MDA) Assay Kit purchased from MedChemExpress. Usage Cited in: Pestic Biochem Physiol. 2025 Sep:213:106563. [Abstract]
Effects of varying chaetoglobosin D concentrations on content in C. mali mycelia (For lipid peroxidation analysis, 100 mg of ground mycelia were homogenized in 1 mL of 10 % (w/v) trichloroacetic acid (TCA) containing 0.1 g quartz sand using a chilled mortar and pestle. The homogenate was centrifuged at 10,000 ×g for 15 min at 4 ◦C, and the supernatant was collected. Malondialdehyde (MDA) content was quantified according to the manufacturer’s instructions of the lipid peroxidation (MDA) assay kit)
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Mol Cell Endocrinol2026 Mar:613:112721. PMID: 41422938
Lipid Peroxidation (MDA) Assay Kit purchased from MedChemExpress. Usage Cited in: Mol Cell Endocrinol. 2026 Mar:613:112721. [Abstract]
Detection of MDA concentration of indicated cells in each group. Malondialdehyde (MDA) content was quantified according to the manufacturer's instructions of the Lipid Peroxidation (MDA) Assay Kit.
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Lipid Peroxidation (MDA) Assay Kit purchased from MedChemExpress. Usage Cited in: Research Square Preprint. 2024 Nov 06.
The content of malondialdehyde (MDA) in Huh7 and Hep3B cells after TTN overexpression and 5-FU treatment. Malondialdehyde (MDA) content was quantified according to the manufacturer's instructions of the Lipid Peroxidation (MDA) Assay Kit.
Storage
-20℃, 1 year
Keep away from light and avoid repeated freezing and thawing
Components
| Components | HY-K0319-100 T |
|---|---|
| MDA Detection Solution | 20 mL |
| Antioxidant | 200 μL |
| MDA Standard Solution (1 mM) | 500 μL |
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