Lipid Peroxidation (MDA) Assay Kit 

Lipid peroxidation generally refers to the oxidative degradation of cellular lipids by reactive oxygen species. Peroxidation of unsaturated lipids affects cell membrane properties, signal transduction pathways, apoptosis, and the deterioration of foods and other biological compounds. Lipid peroxidation degradation forms reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). In this process free radicals take electrons from the lipids (generally in cell membranes), resulting in cell damage. Therefore MDA and 4-HNE are often used as markers of lipid peroxidation, and to assay for oxidative damage / oxidative stress.

MCE Lipid Peroxidation (MDA) Assay Kit is suitable for measuring MDA levels in a variety of samples including plasma, serum, urine, tissues or cell lysates. The detection principle is that the MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct (The reaction principle is illustrated in the following figure). The MDA-TBA adduct can be quantified colorimetrically (OD = 535 nm) fluorescent (Ex/Em = 532/553 nm) detection.

-20℃, 1 year

Keep away from light and avoid repeated freezing and thawing