2. Oligonucleotides
  3. Cap Analogs

Cap Analogs

The “cap” structure (m7G[5']ppp[5']N, where N is any nucleotide) is present at the 5-end of eukaryotic mRNA molecules synthesized by RNA polymerase II. It typically consists of a 7-methylguanosine moiety attached via a 5-5 triphosphate bridge to the 5-terminal nucleoside of the RNA strand. The distinct cap structure plays a vital role in providing resistance to 5′‐exonuclease that helps to protect the mRNA from speedy degradation by 5′‐exonuclease activity. It has participated in numerous aspects of mRNA metabolism such as intracellular transport, splicing, subcellular localization, initiation of RNA, translation, and mRNA turnover. The use of the synthetic dinucleotide analog m7G[5']ppp[5']G has been the most common method for in vitro synthesis of 5-capped mRNAs. The presence of cap structure confers mRNA stability and enables efficient translation.

Cap Analogs (33):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-145974A
    m7GpppAmpG ammonium solution (100 mM) 3080642-79-8 99.96%
    m7GpppAmpG ammonium (m7G(5')ppp(5')(2'OMeA)pG ammonium) is a trinucleotide 5′ end cap analog. m7GpppAmpG ammonium binds to eIF4E with a KD value of 45.6 nM. m7GpppAmpG ammonium caps RNA with a capping efficiency of 90%. m7GpppAmpG ammonium enhances mRNA stability and translation efficiency. m7GpppAmpG ammonium is used in mRNA therapeutic research.
    m7GpppAmpG ammonium solution (100 mM)
  • HY-139100B
    m7G(5')ppp(5')A diammonium 75252-10-7 99.15%
    m7G(5')ppp(5')A (m7GpppA) diammonium is a dinucleotide cap analog that can be used for in vitro RNA transcription.
    m7G(5')ppp(5')A diammonium
  • HY-153108
    3'-O-Me-m7G(5')ppp(5')A solution (100 mM) 99.43%
    3'-O-Me-m7G(5')ppp(5')A (ARCA cap) solution (100 mM), anti-reverse cap analog, has a special RNA cap structure. 3'-O-Me-m7G(5')ppp(5')A solution (100 mM) improves mRNA translation efficiency and stability, reduces translational inhibition by proteins such as IFIT1, and enables stronger and longer-lasting expression of the target protein. The RNA cap structure is a common feature of mRNAs in some RNA viruses and eukaryotes, and it serves as a signal for translation initiation.
    3'-O-Me-m7G(5')ppp(5')A solution (100 mM)
  • HY-145969A
    β-S-ARCA triammonium 99.48%
    β-S-ARCA (3'-O-Me-m7G(5')ppp(5')G) triammonium is a mRNA 7-methylguanosine (m7G) cap analog carrying a phosphorothioate (PS) moiety. β-S-ARCA triammonium binds eIF4E via electrostatic interactions between its β-sulfur atom and positively charged Arg and Lys residues in the protein binding site. β-S-ARCA triammonium prevents the decapping by Dcp2, increases the mRNA half-life, enhances cap-dependent translation, and increases protein expression in cells. β-S-ARCA triammonium has been applied in researching experimental mRNA-based anticancer vaccines.
    β-S-ARCA triammonium
  • HY-145973A
    3'OMe-m7GpppAmpG ammonium solution (100 mM) 98.24%
    3'OMe-m7GpppAmpG ammonium solution (100 mM) is a trinucleotide Cap analogue. 3'OMe-m7GpppAmpG ammonium shows a significant translational efficiency. 3'OMe-m7GpppAmpG ammonium can be used as a potential molecular biology tool in the field of mRNA vaccines and mRNA transfection, such as protein production, gene therapy and anti-cancer immunization.
    3'OMe-m7GpppAmpG ammonium solution (100 mM)
  • HY-145975A
    m7Gpppm6AmpG ammonium solution (100 mM) 98.26%
    m7Gpppm6AmpG ammonium solution (100 mM) is a trinucleotide mRNA 5’ cap analogs. m7Gpppm6AmpG ammonium can be used for RNA synthesis in vitro.
    m7Gpppm6AmpG ammonium solution (100 mM)
  • HY-158772
    m7G(5')ppp(5')(2'OMeA)pU ammonium solution (100 mM) 99.6%
    m7G(5')ppp(5')(2'OMeA)pU ammonium is a cap analog that can be used for in vitro transcription of mRNA.
    m7G(5')ppp(5')(2'OMeA)pU ammonium solution (100 mM)
  • HY-164190
    m7(3'Ma-Biotin)Gppp(2'OMe)ApG ammonium solution (25mM)
    m7(3'Ma-Biotin)Gppp(2'OMe)ApG ammonium is a Biotin-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of Biotin by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.
    m7(3'Ma-Biotin)Gppp(2'OMe)ApG ammonium solution (25mM)
  • HY-164188
    m7(3'Ma-Cy5)Gppp(2'OMe)ApG triammonium 98%
    m7(3'Ma-Cy5)Gppp(2'OMe)ApG ammonium is a Cy5-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of Cy5 by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.
    m7(3'Ma-Cy5)Gppp(2'OMe)ApG triammonium
  • HY-145977A
    m7GpppGmpG ammonium solution (100 mM)
    m7GpppGmpG ammonium is a trinucleotide 5′ cap analog with the capping efficiencies for the obtained RNAs of 86%.
    m7GpppGmpG ammonium solution (100 mM)
  • HY-145969
    β-S-ARCA 1093268-63-3
    β-S-ARCA (3'-O-Me-m7G(5')ppp(5')G) is a mRNA 7-methylguanosine (m7G) cap analog carrying a phosphorothioate (PS) moiety. β-S-ARCA binds eIF4E via electrostatic interactions between its β-sulfur atom and positively charged Arg and Lys residues in the protein binding site. β-S-ARCA prevents the decapping by Dcp2, increases the mRNA half-life, enhances cap-dependent translation, and increases protein expression in cells. β-S-ARCA has been applied in researching experimental mRNA-based anticancer vaccines.
    β-S-ARCA
  • HY-139100
    N7-Methyl-guanosine-5'-triphosphate-5'-adenosine 62828-63-1
    N7-Methyl-guanosine-5'-triphosphate-5'-adenosine (m7GpppA) is a dinucleotide cap analog that can be used for in vitro RNA transcription.
    N7-Methyl-guanosine-5'-triphosphate-5'-adenosine
  • HY-164192A
    m7(3'AcmG)(5')ppp(5')(2'OMeA)pG trisodium solution (100 mM) 98.61%
    m7(3'AcmG)(5')ppp(5')(2'OMeA)pG trisodium, a cap analogue derived from patent WO2023159930A1, can be used for the synthesis of mRNA.
    m7(3'AcmG)(5')ppp(5')(2'OMeA)pG trisodium solution (100 mM)
  • HY-145913A
    m7GpppApG triammonium solution (100 mM) 99.28%
    m7GpppApG triammonium is a trinucleotide mRNA 5' cap analog that can be used for RNA synthesis in vitro.
    m7GpppApG triammonium solution (100 mM)
  • HY-164193
    m7(3'AcmG)(5')ppp(5')(2'OMeA)pU ammonium
    m7(3'AcmG)(5')ppp(5')(2'OMeA)pU ammonium is a cap analogue that requires the T7 promoter with AU as the start sequence for mRNA synthesis.
    m7(3'AcmG)(5')ppp(5')(2'OMeA)pU ammonium
  • HY-164191
    m7(3'Ma-Peg5-FAM)Gppp(2'OMe)ApG ammonium
    m7(3'Ma-Peg5-FAM)Gppp(2'OMe)ApG ammonium is a FAM-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of FAM by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.
    m7(3'Ma-Peg5-FAM)Gppp(2'OMe)ApG ammonium
  • HY-164189
    m7(3'Ma-Cy3)Gppp(2'OMe)ApG ammonium
    m7(3'Ma-Cy3)Gppp(2'OMe)ApG ammonium is a Cy3-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of Cy3 by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.
    m7(3'Ma-Cy3)Gppp(2'OMe)ApG ammonium
  • HY-158819
    m7(3'OMeG)(5')ppp(5')m6(2'OMeA)pG trisodium 2956651-00-4
    m7(3'OMeG)(5')ppp(5')m6(2'OMeA)pG trisodium is a cap analog that can be used for in vitro transcription of mRNA.
    m7(3'OMeG)(5')ppp(5')m6(2'OMeA)pG trisodium
  • HY-150144
    Uracil-m7GpppAmpG ammonium solution (100 mM)
    Uracil-m7GpppAmpG ammonium solution (100 mM) is a cap analog that can be used for mRNA synthesis.
    Uracil-m7GpppAmpG ammonium solution (100 mM)
  • HY-145980
    m7GpppUpG 2638447-85-3
    m7GpppUpG, an oligonucleotide, is an M7GpppNpG trinucleotide cap analogue. m7GpppUpG can be used as a chemical tool enabling manufacturing of RNA featuring either cap 0 or cap 1 structures.
    m7GpppUpG