Cap Analogs

m7GpppAmpG ammonium (m7G(5')ppp(5')(2'OMeA)pG ammonium) is a trinucleotide 5′ end cap analog. m7GpppAmpG ammonium binds to eIF4E with a K_D value of 45.6 nM. m7GpppAmpG ammonium caps RNA with a capping efficiency of 90%. m7GpppAmpG ammonium enhances mRNA stability and translation efficiency. m7GpppAmpG ammonium is used in mRNA therapeutic research.

m7G(5')ppp(5')A (m7GpppA) diammonium is a dinucleotide cap analog that can be used for in vitro RNA transcription.

N7-Methyl-guanosine-5'-triphosphate-5'-adenosine (m7GpppA) is a dinucleotide cap analog that can be used for in vitro RNA transcription.

3'-O-Me-m7G(5')ppp(5')A (ARCA cap) solution (100 mM), anti-reverse cap analog, has a special RNA cap structure. 3'-O-Me-m7G(5')ppp(5')A solution (100 mM) improves mRNA translation efficiency and stability, reduces translational inhibition by proteins such as IFIT1, and enables stronger and longer-lasting expression of the target protein. The RNA cap structure is a common feature of mRNAs in some RNA viruses and eukaryotes, and it serves as a signal for translation initiation.

β-S-ARCA (3'-O-Me-m7G(5')ppp(5')G) triammonium is a mRNA 7-methylguanosine (m7G) cap analog carrying a phosphorothioate (PS) moiety. β-S-ARCA triammonium binds eIF4E via electrostatic interactions between its β-sulfur atom and positively charged Arg and Lys residues in the protein binding site. β-S-ARCA triammonium prevents the decapping by Dcp2, increases the mRNA half-life, enhances cap-dependent translation, and increases protein expression in cells. β-S-ARCA triammonium has been applied in researching experimental mRNA-based anticancer vaccines.

β-S-ARCA (3'-O-Me-m7G(5')ppp(5')G) is a mRNA 7-methylguanosine (m7G) cap analog carrying a phosphorothioate (PS) moiety. β-S-ARCA binds eIF4E via electrostatic interactions between its β-sulfur atom and positively charged Arg and Lys residues in the protein binding site. β-S-ARCA prevents the decapping by Dcp2, increases the mRNA half-life, enhances cap-dependent translation, and increases protein expression in cells. β-S-ARCA has been applied in researching experimental mRNA-based anticancer vaccines.

3’OMe-m7GpppAmpG (m7(3'OMeG)(5')ppp(5')(2'OMeA)pG) ammonium solution (100 mM) is a trinucleotide Cap1 analog with the structure m7 (3'OMeG)(5') ppp (5')(2'OMeA) pG, and also functions as a cis-acting ligase ribozyme inhibitor. 3’OMe-m7GpppAmpG ammonium solution (100 mM) effectively reduces free 5'-triphosphate groups on RNA transcripts, thereby enabling efficient co-transcriptional capping of in vitro transcribed mRNA. 3’OMe-m7GpppAmpG ammonium solution (100 mM) is not only widely used in the preparation of modified mRNA including trivalent influenza vaccine candidates, but also applicable to studies related to SARS-CoV-2 infection and other relevant research.

m7G(5')ppp(5')(2'OMeA)pU ammonium is a cap analog that can be used for in vitro transcription of mRNA.

m7Gpppm6AmpG ammonium solution (100 mM) is a trinucleotide mRNA 5’ cap analogs. m7Gpppm6AmpG ammonium can be used for RNA synthesis in vitro.

m7(3'Ma-Biotin)Gppp(2'OMe)ApG ammonium is a Biotin-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of Biotin by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.

m7(3'Ma-Cy5)Gppp(2'OMe)ApG ammonium is a Cy5-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of Cy5 by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.

m7GpppGmpG ammonium is a trinucleotide 5′ cap analog with the capping efficiencies for the obtained RNAs of 86%.

m7(3'AcmG)(5')ppp(5')(2'OMeA)pG trisodium, a cap analogue derived from patent WO2023159930A1, can be used for the synthesis of mRNA.

m7GpppApG triammonium is a trinucleotide mRNA 5' cap analog that can be used for RNA synthesis in vitro.

m7(3'AcmG)(5')ppp(5')(2'OMeA)pU ammonium is a cap analogue that requires the T7 promoter with AU as the start sequence for mRNA synthesis.

m7(3'Ma-Peg5-FAM)Gppp(2'OMe)ApG ammonium is a FAM-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of FAM by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.

m7(3'Ma-Cy3)Gppp(2'OMe)ApG ammonium is a Cy3-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of Cy3 by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.

m7(3'OMeG)(5')ppp(5')m6(2'OMeA)pG trisodium is a cap analog that can be used for in vitro transcription of mRNA.

Uracil-m7GpppAmpG ammonium solution (100 mM) is a cap analog that can be used for mRNA synthesis.

m7GpppUpG, an oligonucleotide, is an M⁷GpppNpG trinucleotide cap analogue. m7GpppUpG can be used as a chemical tool enabling manufacturing of RNA featuring either cap 0 or cap 1 structures.