3'-O-Me-m7G(5')ppp(5')A solution (100 mM)  (Synonyms: ARCA cap solution (100 mM))

3'-O-Me-m7G(5')ppp(5')A (ARCA cap) solution (100 mM), anti-reverse cap analog, has a special RNA cap structure. 3'-O-Me-m7G(5')ppp(5')A solution (100 mM) improves mRNA translation efficiency and stability, reduces translational inhibition by proteins such as IFIT1, and enables stronger and longer-lasting expression of the target protein. The RNA cap structure is a common feature of mRNAs in some RNA viruses and eukaryotes, and it serves as a signal for translation initiation^[1][2].

ARCA?capped mRNA purified by HPLC has minimal impact on the metabolic activity of mesenchymal stem cells and does not trigger a significant innate immune response, making it the preferred cap structure for in vitro engineering of MSCs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

834.52

C₂₂H₃₇N₁₂O₁₇P₃

Liquid

Colorless to light yellow

O[C@@H]1[C@H](OC)[C@@H](COP(OP(OP(OC[C@@H]2[C@@H](O)[C@@H](O)[C@H](N3C(N=CN=C4N)=C4N=C3)O2)([O-])=O)([O-])=O)([O-])=O)O[C@H]1N5C(N=C(N)NC6=O)=C6[N+](C)=C5.[NH4+].[NH4+]

Shipping with dry ice.

Solution, -20°C, 1 year

• [1]. Hadas Y, et al. Optimizing Modified mRNA In Vitro Synthesis Protocol for Heart Gene Therapy. Mol Ther Methods Clin Dev. 2019 Jul 30;14:300-305.  [Content Brief]

  [2]. Andrzejewska A, et al. Mesenchymal stem cell engineering by ARCA analog-capped mRNA. Mol Ther Nucleic Acids. 2023;33:454-468. Published 2023 Jul 17.