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  5. Basement Membrane Matrix
  6. Basement Membrane Matrix GFR (Phenol Red)

Basement Membrane Matrix GFR (Phenol Red) 

Cat. No.: HY-K6003
Manual COA SDS Technical Support
Organoid Culture

MCE Basement Membrane Matrix GFR (Growth Factor Reduced, Phenol Red) is primarily composed of a natural basement membrane matrix derived from mouse tumors and contains lower levels of various cytokines than the standard type. This product is mainly used for organoid culture and for research applications with high requirements for basement membrane preparation.

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    MedChemExpress Validation
    Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
    Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
    Matrix gel: 8 μL
    Complete culture medium: 50 μL
    Under identical tube formation assay conditions, MCE matrix gel (Basement Membrane Matrix GFR (Phenol Red), HY-K6003) demonstrated superior tube-forming performance compared with competitor brands at both undiluted and 2:1 dilution conditions. The tubular structures formed with MCE matrix gel were more complete and continuous, with clearer and more stable network architecture.

    MedChemExpress Validation
    Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
    Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
    Matrix gel: 8 μL
    Complete culture medium: 50 μL
    Under identical tube formation assay conditions, the tube formation effects of different batches of MCE Matrigel (Basement Membrane Matrix GFR (Phenol Red), HY-K6003) were compared under both undiluted and 2:1 diluted conditions.Different batches of MCE Matrigel exhibited consistent tube formation ability. The formed tubular structures were intact and continuous, with clear and stable network structures.The overall tube formation effect was stable and reproducible, with minor differences between batches, indicating good batch-to-batch stability.

    MedChemExpress Validation
    Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
    Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
    Matrix gel: 8 μL
    Complete culture medium: 50 μL
    Under identical tube formation assay conditions, the tube formation effects of MCE Matrigel (Basement Membrane Matrix GFR (Phenol Red), HY-K6003) and competitor brand Matrigel were compared under both undiluted and 2:1 diluted conditions. MCE Matrigel exhibited superior tube formation ability, with more intact and continuous tubular structures and clearer, more stable network structures.

    • Description

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    MCE Basement Membrane Matrix GFR (Growth Factor Reduced, Phenol Red) is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc. The percentage of each of these growth factors is lower than that of standard basement membrane matrix.

    MCE Basement Membrane Matrix GFR (Growth Factor Reduced, Phenol Red) reduces the background signal induced by growth factors and is suitable for organoid culture and for studies with high requirements for basement membrane preparation.
    Storage

    -20°C, 2 years.

    Avoid repeated freezing and thawing.

    Shipping with dry ice
    Protocol

    a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

    b. Use pre-cooled serum-free medium to dilute according to experimental requirements. Refer to the Experimental Conditions table for final concentrations.

    c. Place the culture plate on ice and inject the substrate gel dilution slowly against the wall to avoid air bubbles. Refer to the Experimental Conditions table for the volume injected.

    d. Transfer the culture plate to 37℃ incubator gently. After gelling, aspirate off excess supernatant. Refer to the Experimental Conditions table for gel time.

     

    Note: When thawing the product for the first time, immerse the vial in crushed ice and place it in a 4°C to melt. After melting, mix thoroughly before aliquoting, and then store at -20°C.
    Components
    Components HY-K6003-5 mL HY-K6003-10 mL
    Basement Membrane Matrix GFR (Phenol Red) 5 mL 10 mL
    Documentation

    Manual (207 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Matrix-GelMatrigelExtracellular matrixscaffolds

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