Tris-Glycine-SDS Powder (1 L of 1×) 

Tris-Glycine-SDS (TGS) is widely used as an electrophoresis buffer for SDS-PAGE and Western Blot.

MCE Tris-Glycine-SDS Powder (1 L of 1×) consists of Tris, Glycine and SDS. Each pouch can be diluted to 1 L 1× TGS buffer, in which the concentration of Tris is 25 mM, the concentration of Glycine is 192 mM and the concentration of SDS is 0.1%. This product provides a convenient way to make TGS buffer and eliminates the need to weigh and mix individual components.

Store at room temperature for 3 years.

1. Add 1 pouch of Tris-Glycine-SDS Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2. Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TGS buffer.

Note: The pH of the 1× TGS buffer is 8.3 ± 0.2 @ 25°C.