2. MCE Kits
  3. Protein Biology
  4. Protein Electrophoresis & WB
  5. Electrophoresis Buffer
  6. Tris-Glycine-SDS Powder (1 L of 1×)

Tris-Glycine-SDS Powder (1 L of 1×) 

Cat. No.: HY-K1020
Manual COA SDS Technical Support
Expression Purification

MCE Tris-Glycine-SDS Powder (1 L of 1×) consists of Tris, Glycine and SDS, and is widely used as an electrophoresis buffer for SDS-PAGE and Western Blot. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Tris-Glycine-SDS (TGS) is widely used as an electrophoresis buffer for SDS-PAGE and Western Blot.

MCE Tris-Glycine-SDS Powder (1 L of 1×) consists of Tris, Glycine and SDS. Each pouch can be diluted to 1 L 1× TGS buffer, in which the concentration of Tris is 25 mM, the concentration of Glycine is 192 mM and the concentration of SDS is 0.1%. This product provides a convenient way to make TGS buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1. Add 1 pouch of Tris-Glycine-SDS Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2. Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TGS buffer.

Note: The pH of the 1× TGS buffer is 8.3 ± 0.2 @ 25°C.

Components
Components HY-K1020-10 pouches HY-K1020-50 pouches
Tris-Glycine-SDS Powder (1 L of 1×) 10 pouches 5 × 10 pouches
Documentation

Manual (94 KB) SDS (173 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TRIS-glycine-SDS running bufferTRIS-glycine-SDS Instant Granulesprotein electrophoresis

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