3-Color Prestained Protein Marker (10-190 kDa)
Based on 4 publication(s) in Google Scholar
MCE 3-Color Prestained Protein Marker (10-190 kDa) is a three-color protein standard with 10 prestained proteins ranging from 10 kDa to 190 kDa. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
Store at -20°C, 1 year. Avoid repetitive freeze-thaw cycles while using.
In-stock
(In Japan)
* Please select Quantity before adding items.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
This product is a controlled substance, please contact local distributor for purchase. This product is a controlled substance and not for sale in your territory.
This product is not for sale in your territory.
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HY-K1005
Ultra High Sensitivity ECL Kit -
HY-K0010
Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) -
HY-K1100
SDS-PAGE Sample Loading Buffer (4×) -
HY-K1001
RIPA Lysis Buffer (Strong) -
HY-K2005
High Sensitivity ECL Kit -
HY-K1027
Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) -
HY-K0021
Phosphatase Inhibitor Cocktail I (100× in DMSO) -
HY-K1025
TBS-T Powder (1 L of 1×) -
HY-K0301
Cell Counting Kit-8 -
HY-K0022
Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) -
HY-Y0320
Dimethyl sulfoxide, meets analytical specification of Ch.P. -
HY-K1012
Serum/Protein-Free Cell Freezing Medium
Description & Advantages
MCE 3-Color Prestained Protein Marker has 8 blue-stained bands and 2 reference bands (one green and one orange band at 23 kDa and 65 kDa respectively). This Protein Marker is designed for monitoring protein separation during SDS-PAGE, confirming Western blot transfer efficiency on membranes (PVDF or nitrocellulose membrane) and for approximating the size of target proteins on the gel or blot.
MCE 3-Color Prestained Protein Marker is supplied in gel loading buffer and is ready to use, with no heating, diluting or additional reducing agent necessary.
Protocol
1. Thaw: Thaw 3-Color Prestained Protein Marker (10-190 kDa) at room temperature for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix: Mix thoroughly to ensure the solution is homogeneous.
3. Loading: Load 5 μL of 3-Color Prestained Protein Marker per well for SDS-PAGE.
Note: The marker is supplied in gel loading buffer and is ready to use. No heating, dilution, or additional reducing agent is required. Prestained proteins may show different mobilities and apparent molecular weights in different SDS-PAGE buffer systems.
Publications
-
Journal Impact Factor
-
Most Recent
-
J Control Release2025 Oct 14;388(Pt 1):114337. PMID: 41101699
-
Bio Protoc2025 Aug 5;15(15):e5401. PMID: 40799477
Storage
Store at -20°C, 1 year.
Avoid repetitive freeze-thaw cycles while using.
Components
| Cat. No. | Product Name | Package |
|---|---|---|
| HY-K1011-250 μL | 3-Color Prestained Protein Marker (10-190 kDa) | 250 μL |
| HY-K1011-500 μL | 3-Color Prestained Protein Marker (10-190 kDa) | 250 μL × 2 |
| HY-K1011-1 mL | 3-Color Prestained Protein Marker (10-190 kDa) | 250 μL × 4 |
Documentation
Help & FAQs
How should lyophilized recombinant proteins be reconstituted and stored?
1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.