Human IgG1 Antibody (YA7187)
Human IgG1 is a non-conjugated and rabbit origined monoclonal antibody, targeting to Human IgG1. It can be used as an isotype control.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:1000-2000 |
Product Details
Human IgG1 is a non-conjugated and rabbit origined monoclonal antibody, targeting to Human IgG1. It can be used as an isotype control.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
A synthesized peptide derived from human Human IgG1 : 90-120
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Human IgG1 is a Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens[1][2].
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Subcellular Localization
Isoform 1 Secreted; Isoform 2 Cell membrane
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Isoforms & Post-Translational Modification
Human (P01857) has 2 isomers: P01857-1: 36,106 Da (predicted); P01857-2: 43,912 Da (predicted).
Human (P01859) has two isomers: P01859-1: 35,901 Da (predicted); P01859-2: 43,806 Da (predicted).
Human (P01860) has two isomers: P01860-1: 41,287 Da (predicted); P01860-2: 49,093 Da (predicted).
Human (P01861) has two isomers: P01861-1: 35,941 Da (predicted); P01861-2: 43,832 Da (predicted).
P01857: Glycosylation on Asn-180 is required for interaction with Fc receptors and ability to activate the complement pathway.
P01859: Glycosylation on Asn-176 is required for interaction with Fc receptors and ability to activate the complement pathway.
P01860: N-linked glycans at Asn-322 are noncore fucosylated and the vast majority are diantennary species with a bisecting GlcNAc.
P01861: Glycosylation on Asn-177 is required for interaction with Fc receptors and ability to activate the complement pathway. -
Subunit
P01857: Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked (PubMed:20176268). Interacts with FCGR1A; this interaction mediates IgG effector functions on monocytes. Interacts with FCGR2A and FCGR3A (PubMed:11711607).
P01859, P01860 and P01861: Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked.
Documentation
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