Human IgG1 Antibody (YA7187)

Cat. No.: HY-P87502: Data Sheet User Guide for Antibodies
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Human IgG1 is a non-conjugated and rabbit origined monoclonal antibody, targeting to Human IgG1. It can be used as an isotype control.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Human IgG1 is a non-conjugated and rabbit origined monoclonal antibody, targeting to Human IgG1. It can be used as an isotype control.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 44 kDa;

Observed band size: 52 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P01857 / P01859 / P01860 / P01861

Gene ID

3500 [NCBI]

Immunogen

A synthesized peptide derived from human Human IgG1 : 90-120

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000

Purity	affinity purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414).

Subcellular Localization：Isoform 1 Secreted; Isoform 2 Cell membrane

Isoforms & Post-Translational Modification：Human (P01857) has 2 isomers: P01857-1: 36,106 Da (predicted); P01857-2: 43,912 Da (predicted).
Human (P01859) has two isomers: P01859-1: 35,901 Da (predicted); P01859-2: 43,806 Da (predicted).
Human (P01860) has two isomers: P01860-1: 41,287 Da (predicted); P01860-2: 49,093 Da (predicted).
Human (P01861) has two isomers: P01861-1: 35,941 Da (predicted); P01861-2: 43,832 Da (predicted).
P01857: Glycosylation on Asn-180 is required for interaction with Fc receptors and ability to activate the complement pathway.
P01859: Glycosylation on Asn-176 is required for interaction with Fc receptors and ability to activate the complement pathway.
P01860: N-linked glycans at Asn-322 are noncore fucosylated and the vast majority are diantennary species with a bisecting GlcNAc.
P01861: Glycosylation on Asn-177 is required for interaction with Fc receptors and ability to activate the complement pathway.

Subunit：P01857: Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked (PubMed:20176268). Interacts with FCGR1A; this interaction mediates IgG effector functions on monocytes. Interacts with FCGR2A and FCGR3A (PubMed:11711607).
P01859, P01860 and P01861: Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked.

Documentation

User Guide for Antibodies (1077 KB)