Tris-HEPES-SDS Powder (500 mL of 1×) 

Tris-HEPES-SDS (HEPES) is widely used in protein electrophoresis, as well as in biochemical diagnostic kits, DNA/RNA extraction kits, PCR diagnostic kits, etc. HEPES is non-toxic to cells.

MCE Tris-HEPES-SDS Powder (500 mL of 1×) consists of Tris, HEPES, EDTA and SDS. Each pouch can be diluted to 500 mL 1× HEPES buffer, in which the concentration of Tris is 50 mM, the concentration of HEPES is 50 mM, the concentration of EDTA is 2 mM and the concentration of SDS is 0.1%. This product provides a convenient way to make HEPES buffer and eliminates the need to weigh and mix individual components.

Store at room temperature for 3 years.

1.   Add 1 pouch of Tris-HEPES-SDS Powder into the cleaned beaker, dissolve with 300 mL distilled water under a magnetic stirrer.

2.   Add distilled water to the solution in step 1 until the total volume is 500 mL. The final solution is 1× HEPES buffer.

Note: The pH of the 1× HEPES buffer is 7.7 ± 0.2 @ 25°C.